High intensity focused ultrasound (HIFU) technology is becoming a potential noninvasive treatment for solid tumor. To explore whether HIFU can be applied to treat melanoma and its metastasis, we investigated the effect of HIFU on murine melanoma model. While there was little influence on cell survival, viability or apoptosis, HIFU exposure suppressed melanoma cell migration in vitro and metastasis in vivo. The expression of microRNA-21(miR-21) was down-regulated and PTEN expression was up-regulated in response to HIFU exposure, which was in concomitant with the reduction of AKT activity. Furthermore, ectopic miR-21 expression suppressed this effect of HIFU. These results demonstrate that HIFU exposure can inhibit AKT-mediated melanoma metastasis via miR-21 inhibition to restore PTEN expression. Therefore, targeting the miR-21/PTEN/AKT pathway might be a novel strategy of HIFU in treatment of melanoma.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226595 | PMC |
| http://dx.doi.org/10.18632/oncotarget.10433 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Intraoperative HIFU ablation of the liver at the hepatocaval confluence as adjunct to surgery: Preliminary animal experiments.
Ultrasonics
December 2024
LabTAU, INSERM, Centre Leon Berard, Universite Lyon 1, F-69003 LYON, France. Electronic address:
Treating colorectal liver metastases (CLMs) located at the hepatocaval confluence with surgery is challenging due to its complexity and associated high risks of perioperative mortality and morbidity. Moreover, thermal ablation techniques are sensitive to the "heat-sink" effect, which reduces their efficacy when tumors are in contact with major blood vessels. In this study we evaluated the feasibility and safety of an intraoperative high-intensity focused ultrasound (HIFU) device for destroying liver tissue volumes sufficiently large to consider treating CLMs at the hepatocaval confluence.
View Article and Find Full Text PDFBoiling Histotripsy in Ex Vivo Human Brain: Proof-of-concept.
Ultrasound Med Biol
February 2025
Physics Faculty, Lomonosov Moscow State University, Moscow, Russia.
Objective: Non-invasive surgical approaches, including boiling histotripsy (BH), are currently being developed for the treatment of brain disorders aiming to avoid craniotomy and exposure of intervening tissues, and, thus, minimize associated complications. This work aimed to demonstrate the feasibility of BH for mechanical fractionation of human brain tissues ex vivo under B-mode guidance, with preliminary measurements of tissue stiffness via shear wave elastography.
Methods: Young's moduli of 25 human autopsy brain samples obtained from de-identified patients of 51-91 y old (median 77 y old) were measured via shear wave elastography prior to BH sonication.
Continuous transcranial ultrasound in large vessel stroke: Image guidance for high-intensity focused sonothrombolysis.
J Neuroimaging
November 2024
Department of Medicine, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
Background And Purpose: Sonothrombolysis is a potential adjunctive therapy for large vessel occlusion (LVO) stroke. Bedside ultrasound image-guided high-intensity focused ultrasound (HIFU) therapy could deliver higher energy therapeutic ultrasound to the thrombus with higher precision than what was previously accomplished in human trials. The aim is to test the feasibility of diagnostic transcranial contrast-enhanced ultrasound (CEUS) to image the occlusion site and continuously maintain the guidance image on-target for a sufficient exposure time for HIFU to be effective during LVO stroke evaluation and treatment.
View Article and Find Full Text PDFA comprehensive numerical procedure for high-intensity focused ultrasound ablation of breast tumour on an anatomically realistic breast phantom.
PLoS One
October 2024
Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
High-Intensity Focused Ultrasound (HIFU) as a promising and impactful modality for breast tumor ablation, entails the precise focalization of high-intensity ultrasonic waves onto the tumor site, culminating in the generation of extreme heat, thus ablation of malignant tissues. In this paper, a comprehensive three-dimensional (3D) Finite Element Method (FEM)-based numerical procedure is introduced, which provides exceptional capacity for simulating the intricate multiphysics phenomena associated with HIFU. Furthermore, the application of numerical procedures to an anatomically realistic breast phantom (ARBP) has not been explored before.
View Article and Find Full Text PDFOptimizing high-intensity focused ultrasound-induced immunogenic cell-death using passive cavitation mapping as a monitoring tool.
J Control Release
November 2024
Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium; Cancer Research Institute Ghent (CRIG), 9000 Ghent, Belgium; Ghent Research Group on Nanomedicines, Faculty of Pharmaceutical Sciences, Ghent University, 9000 Ghent, Belgium.
Over the past decade, ultrasound (US) has gathered significant attention and research focus in the realm of medical treatments, particularly within the domain of anti-cancer therapies. This growing interest can be attributed to its non-invasive nature, precision in delivery, availability, and safety. While the conventional objective of US-based treatments to treat breast, prostate, and liver cancer is the ablation of target tissues, the introduction of the concept of immunogenic cell death (ICD) has made clear that inducing cell death can take different non-binary pathways through the activation of the patient's anti-tumor immunity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!