Frankia sp. strain EI5c is a member of Frankia lineage III, which is able to reinfect plants of the Eleagnaceae, Rhamnaceae, Myricaceae, and Gymnostoma, as well as the genus Alnus Here, we report the 6.6-Mbp draft genome sequence of Frankia sp. strain EI5c with a G+C content of 72.14 % and 5,458 candidate protein-encoding genes.
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http://dx.doi.org/10.1128/genomeA.00660-16 | DOI Listing |
Int Microbiol
December 2024
Department of Biological and Chemical Engineering, USCR Molecular Bacteriology and Genomics, National Institute of Applied Science and Technology, University of Carthage, 1080, Tunis Cedex, Tunisia.
This study re-evaluates Pseudofrankia strains, traditionally regarded as parasitic dwellers of actinorhizal root nodules due to their inability to fix nitrogen (Fix -) and/or nodulate (Nod -), as potential plant growth-promoting bacteria (PGPB). We compared plant growth-promoting traits (PGPTs) between Pseudofrankia strains, including one newly sequenced strain BMG5.37 in this study and typical (Fix + /Nod +) Frankia, Protofrankia, and Parafrankia, as well as non-frankia actinorhizal species Nocardia and Micromonospora, and the phytopathogenic Streptomyces.
View Article and Find Full Text PDFSci Data
December 2024
Department of Plant Sciences, University of California, Davis, CA, USA.
There have been frequent reports of more than one strain of the nitrogen-fixing symbiont, Frankia, in the same root nodule of plants in the genus Alnus, but quantitative assessments of their relative contributions have not been made to date. Neither has the diversity of other microbes, having potential functional roles in symbiosis, been systematically evaluated. Alnus rubra root nodule microbiota were studied using Illumina short read sequencing and kmer-based read classification.
View Article and Find Full Text PDFJ Exp Bot
November 2024
Department of Ecology, Environment and Plant Science, Stockholm University, 106 91 Stockholm, Sweden.
Frankia cluster-2 strains are diazotrophs that engage in root nodule symbiosis with actinorhizal plants of the Cucurbitales and the Rosales. Previous studies have shown that an assimilated nitrogen source, presumably arginine, is exported to the host in nodules of Datisca glomerata (Cucurbitales), while a different metabolite is exported in the nodules of Ceanothus thyrsiflorus (Rosales). To investigate if an assimilated nitrogen form is commonly exported to the host by cluster-2 strains, and which metabolite would be exported in Ceanothus, we analysed gene expression levels, metabolite profiles, and enzyme activities in nodules.
View Article and Find Full Text PDFFEMS Microbiol Ecol
November 2024
Department of Plant Physiology, UPSC, Umeå University, 90187 Umeå, Sweden.
Uptake hydrogenase (Hup) recycles H2 formed by nitrogenase during nitrogen fixation, thereby preserving energy. Among root nodule bacteria, most rhizobial strains examined are Hup-, while only one Hup- Frankia inoculum had been identified. Previous analyses had led to the identification of two different [NiFe] hydrogenase syntons.
View Article and Find Full Text PDFFront Microbiol
July 2024
LR Pastoral Ecology, Arid Regions Institute, University of Gabes, Medenine, Tunisia.
Nearly 50 years after the ground-breaking isolation of the primary microsymbiont under axenic conditions, efforts to isolate a substantial number of and strains continue with enduring challenges and complexities. This study aimed to streamline genomic insights through comparative and predictive tools to extract traits crucial for isolating specific in axenic conditions. Pangenome analysis unveiled significant genetic diversity, suggesting untapped potential for cultivation strategies.
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