AI Article Synopsis
- The study aimed to examine how osteoprotegerin (OPG) impacts the activity of a specific type of bone cell, known as cementoblasts, using a mouse cell line in a lab setting.
- Researchers used various techniques, including cell transfection and assays, to confirm that OPG was successfully introduced into the cells and to measure any changes in cell growth and gene expression.
- Results showed that increasing OPG levels led to a greater proliferation rate of the cementoblasts and enhanced expression of key genes and proteins associated with bone formation, indicating that OPG boosts cementoblast activity.
Article Abstract
Objective: To investigate the effect of osteoprotegerin (OPG) on the cementoblastic activity of a clonal population of immortalised murine cementoblasts (OCCM-30) in vitro.
Methods: OCCM-30 cells were transiently transfected with the mouse OPG using the Avalanche transfection reagent. The ectopic expression of OPG was confirmed by enzyme-linked immunosorbent assay and quantitative polymerase chain reaction. The cell counting Kit-8 assay was used to investigate the effect of OPG on cell proliferation. The expression levels of cementoblastic-related mRNA and protein in the transfected OCCM-30 cells were detected using real-time PCR, Western blotting and immunohistochemical staining.
Results: Satisfactory transfection efficiency was observed 48 h after transfection. The results of the cell proliferation assay indicated that the expansion rate of the OPG transfection group was greater than that of the control group at both 72 h and 96 h. The mRNA levels of osterix (Osx), protein kinase B (Akt1), cementum attachment protein (CAP) and osteopontin (Opn) were significantly upregulated (P < 0.05) in the OPG group. Protein levels of OPN, bone sialoprotein II (BSP II), osteocalcin (OC) and CAP, which are responsible for osteogenetic and cementoblastic activity, were significantly increased in the OPG-overexpressing group.
Conclusion: Overexpression of OPG in OCCM-30 cells promotes cementoblastic activity.
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| http://dx.doi.org/10.3290/j.cjdr.a36180 | DOI Listing |
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