[Down-regulation of TIPE2 promotes the proliferation and immune activity of T lymphocytes].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi

Department of Thoracic Surgery, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China. *Corresponding author, E-mail:

Published: July 2016

Objective To utilize specific small interfering RNA (siRNA) to silence the expression of tumor necrosis factor α-induced protein 8 like-2 (TIPE2) gene of T lymphocytes and investigate the effect of TIPE2 targeting siRNA on T lymphocyte proliferation and immune function. Methods Mouse spleen T lymphocytes were sorted by magnetic beads. Western blotting was used to screen and validate an effective siRNA to silence the TIPE2 gene expression of T lymphocytes. Twenty-four hours after transfection with the siRNA into T lymphocytes, the expression of CD69 in each group was detected by flow cytometry. Seventy-two hours after transfection, the proliferation of the T lymphocytes was measured with CCK-8 assay; meanwhile, the secretion levels of interleukin 2 (IL-2) and interferon γ (IFN-γ) in each group were measured by ELISA. Results We obtained TIPE2 targeting siRNA sequences and effectively silenced the expression of TIPE2 gene. After TIPE2 gene expression was down-regulated, the expression of the CD69 on T lymphocytes increased, and the proliferation of T lymphocytes and the secretion of IL-2 and IFN-γ were enhanced. Conclusion Down-regulation of TIPE2 gene expression can promote the T lymphocyte proliferation and immune activity.

Download full-text PDF

Source

Publication Analysis

Top Keywords

tipe2 gene
20
proliferation immune
12
gene expression
12
immune activity
8
sirna silence
8
tipe2 targeting
8
targeting sirna
8
lymphocyte proliferation
8
hours transfection
8
expression cd69
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!