We studied the effects of different variables on luminol-enhanced chemiluminescence (CL) of whole blood, induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP), opsonized zymosan particles (OZP), or phosphate buffered saline (PBS). Use of correction factor based on blood neutrophil count and hemoglobin concentration decreased interindividual variation and improved normality of the frequency distributions of CL responses. In FMLP- and PBS-induced CL, day-to-day variation was not significant, but it was significant in OZP-induced CL. Although storage of blood samples for 8 hours decreased CL, the decrease was not evident in a study of initial activation, i.e. CL measured at one minute after application of FMLP. Our results suggest that FMLP-induced initial activation is well suited for epidemiological studies because it is easy to determine and is affected neither by day-to-day variation nor by storage of the blood samples for a few hours.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Non-Contact Interaction Between Phorbol Myristate Acetate and Aqueous Alcohol Solutions Under Combined Magnetic Fields.
Molecules
December 2024
Faculty of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
Previous research has demonstrated that a combined magnetic field (CMF) plays a critical role in modifying the properties of aqueous solutions, leading to an increase in the luminol-enhanced chemiluminescence of neutrophils. Using this model, the distant interaction between aqueous solutions was demonstrated, and the role of a CMF in the regulation of this phenomenon was established. In the current study, highly diluted (HD) phorbol myristate acetate (PMA) solution (the donor) was incubated with aqueous ethanol (the acceptor), both in a CMF-generating device and under geomagnetic field (GMF), for 0, 20, and 60 min.
View Article and Find Full Text PDFSynthesis and anti-inflammatory activity of benzimidazole derivatives; an in vitro, in vivo and in silico approach.
Heliyon
May 2024
Department of Pharmaceutical Chemistry, Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad, Pakistan.
Many non-steroidal anti-inflammatory drugs (NSAIDs) concurrently inhibit both COX-1 and COX-2, with a preference for specifically targeting COX-2 due to its significant involvement in various pathologies. In addition to COX enzymes, several other targets, including Aldose reductase, Aldo-ketoreductase family 1-member C2, and Phospholipase A2, have been identified as contributors to inflammation and a myriad of other diseases. In this context, a series of 2-substituted benzimidazole derivatives was synthesized and assessed for their anti-inflammatory potential through both in vitro and in vivo assays.
View Article and Find Full Text PDFHuman Neutrophils Couple Nitric Oxide Production and Extracellular Trap Formation in Allergic Asthma.
Am J Respir Crit Care Med
September 2024
Facultad de Odontología, Universidad de Sevilla, Seville, Spain.
Nitric oxide (NO) is increased in the airways and serum of patients with allergic asthma, suggesting an important role in asthma. NO production has been widely attributed to the canonical inducible NO synthase. Much effort has been made to inhibit this enzyme, with two outcomes: no asthma improvement and partial NO reduction, suggesting the involvement of an inducible NO synthase-independent source.
View Article and Find Full Text PDFOxidative metabolism of neutrophils in acute coronary syndrome.
Cardiovasc J Afr
July 2023
Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia; Demikhov City Clinical Hospital, Moscow, Russia.
Background: Inflammation in acute coronary syndrome (ACS) involves neutrophil activation and oxidative stress. Here, we studied the production of reactive oxygen species (ROS) by neutrophils in ACS.
Methods: The study included 42 patients, men and women aged 46-91 years with ischaemic heart disease (IHD), non-ST-segment elevation ACS and ST-segment elevation ACS.
Pharmacological effects of Raphanus caudatus in inflammation and hematopoitic system: an in vitro and in vivo investigation.
Pak J Pharm Sci
November 2022
Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Karachi, Pakistan.
The pods of Radish are known as Raphanus caudatus that belongs to the family Brassicaceae. They are commonly known as Mungra or Sungra in Pakistan, while the common English name for this species is Rat-tailed radish. This variety of radish is unique and less tested for pharmacological as well as toxicological potential.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!