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Detection of human coronavirus RNA in surgical smoke generated by surgical devices.
J Hosp Infect
November 2021
Department of Obstetrics and Gynecology, Kansai Medical University, Osaka, Japan.
Background: Gaseous by-products generated by surgical devices - collectively referred to as 'surgical smoke' - present the hazard of transmitting infective viruses from patients to surgical teams. However, insufficient evidence exists to evaluate and mitigate the risks of SARS-CoV-2 transmission via surgical smoke.
Aim: To demonstrate the existence and infectivity of human coronavirus RNA in surgical smoke using a model experiment and to evaluate the possibility of lowering transmission risk by filtration through a surgical mask.
Isolation of Human Coronaviruses OC43, HKU1, NL63, and 229E in Yamagata, Japan, Using Primary Human Airway Epithelium Cells Cultured by Employing an Air-Liquid Interface Culture.
Jpn J Infect Dis
July 2021
Department of Microbiology, Yamagata Prefectural Institute of Public Health, Japan.
Article Synopsis
- Isolating seasonal coronaviruses, such as HCoV-OC43 and HCoV-NL63, is challenging but can be effectively achieved using a modified air-liquid interface (ALI) culture method.
- Researchers in Yamagata, Japan isolated 29 HCoVs from nasopharyngeal specimens, demonstrating the potential of ALI cultures to produce a high yield of viral genome copies over an extended period.
- The study also showed that HCoV-NL63 can be successfully propagated in the LLC-MK2 cell line, suggesting these methods could aid in future large-scale culturing and research on seasonal coronaviruses.
HeLa-ACE2-TMPRSS2 Cells Are Useful for the Isolation of Human Coronavirus 229E.
Jpn J Infect Dis
September 2016
Department of Microbiology, Yamagata Prefectural Institute of Public Health.
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