AI Article Synopsis
- The text discusses the challenge of creating predictive models for plant organ growth, focusing on the need to connect molecular and organ scales quantitatively.
- A new method is introduced for analyzing growth patterns in rod-shaped plant organs like roots and hypocotyls, which does not rely on surface markers.
- The method, named KymoRod, enables detailed examination of growth dynamics in Arabidopsis thaliana under various conditions and is available as a user-friendly Matlab tool.
Article Abstract
A major challenge in plant systems biology is the development of robust, predictive multiscale models for organ growth. In this context it is important to bridge the gap between the, rather well-documented molecular scale and the organ scale by providing quantitative methods to study within-organ growth patterns. Here, we describe a simple method for the analysis of the evolution of growth patterns within rod-shaped organs that does not require adding markers at the organ surface. The method allows for the simultaneous analysis of root and hypocotyl growth, provides spatio-temporal information on curvature, growth anisotropy and relative elemental growth rate and can cope with complex organ movements. We demonstrate the performance of the method by documenting previously unsuspected complex growth patterns within the growing hypocotyl of the model species Arabidopsis thaliana during normal growth, after treatment with a growth-inhibiting drug or in a mechano-sensing mutant. The method is freely available as an intuitive and user-friendly Matlab application called KymoRod.
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| http://dx.doi.org/10.1111/tpj.13255 | DOI Listing |
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