Background: Quantification of target analyte by LC-MS/MS is sometimes hampering due to competitive adduct ions formation (sodium and/or ammonium) in positive ionization mode. A UPLC-MS/MS assay was developed for the determination of apremilast in rat plasma using ESI-negative mode to avoid adduct ions formation.
Method & Results: After extraction from plasma by ethyl acetate, analyte and IS were separated on Aquity BEH C column using acetonitrile-10 mM ammonium acetate (85:15) as mobile phase. The calibration curve was linear between 3.04 and 1000 ng/ml with correlation coefficients (r) of ≥0.995 and lower limit of quantification of 3.04 ng/ml. All validation parameter results were within the acceptable range. The assay was successfully employed in oral PK study with C of 584.29 ng/ml and AUC of 6530 ng.h/ml after apremilast (2 mg/kg) administration.
Conclusion: This result suggests that ESI in negative mode would be an alternative approach for LC-MS/MS quantification of analytes, which produce competitive adducts in positive mode.
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