AI Article Synopsis
- The midgut of insects, particularly in Lepidoptera like the silkworm, is a valuable model for studying intestinal stem cells (ISCs) and processes like apoptosis and autophagy.
- During larva-larva molting, larvae experience nutrient starvation and significant organ changes, making this phase critical for ISC analysis.
- This study focuses on the silkworm's midgut during this molting phase, examining ISC behavior and metabolic changes, which offers insights into the complex functions of the midgut epithelium.
Article Abstract
The midgut of insects has attracted great attention as a system for studying intestinal stem cells (ISCs) as well as cell death-related processes, such as apoptosis and autophagy. Among insects, Lepidoptera represent a good model to analyze these cells and processes. In particular, larva-larva molting is an interesting developmental phase since the larva must deal with nutrient starvation and its organs are subjected to rearrangements due to proliferation and differentiation events. Several studies have analyzed ISCs in vitro and characterized key factors involved in their division and differentiation during molt. However, in vivo studies performed during larva-larva transition on these cells, and on the whole midgut epithelium, are fragmentary. In the present study, we analyzed the larval midgut epithelium of the silkworm, Bombyx mori, during larva-larva molting, focusing our attention on ISCs. Moreover, we investigated the metabolic changes that occur in the epithelium and evaluated the intervention of autophagy. Our data on ISCs proliferation and differentiation, autophagy activation, and metabolic and functional activities of the midgut cells shed light on the complexity of this organ during the molting phase.
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| http://dx.doi.org/10.1016/j.asd.2016.06.002 | DOI Listing |
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