AI Article Synopsis
- The study introduces a new method called plasmon coupling enhanced Raman scattering (PCERS) nanobeacon for highly sensitive detection of cholera toxin (CT) in a single step.
- The method utilizes specially designed plasmonic nanoparticles, which have a bilayer phospholipid coating containing Raman indicators and CT-binding ligands for optimal interaction with CT.
- It shows significant enhancement of Raman signals during detection, indicating potential for practical use in rapid and accurate testing for cholera in various settings.
Article Abstract
We report the development of a novel plasmon coupling enhanced Raman scattering (PCERS) method, PCERS nanobeacon, for ultrasensitive, single-step, homogeneous detection of cholera toxin (CT). This method relies on our design of the plasmonic nanoparticles, which have a bilayer phospholipid coating with embedded Raman indicators and CT-binding ligands of monosialoganglioside (GM1). This design allows a facile synthesis of the plasmonic nanoparticle via two-step self-assembly without any specific modification or chemical immobilization. The realization of tethering GM1 on the surface imparts the plasmonic nanoparticles with high affinity, excellent specificity, and multivalence for interaction with CT. The unique lipid-based bilayer coated structure also affords excellent biocompatibility and stability for the plasmonic nanoparticles. The plasmonic nanoparticles are able to show substantial enhancement of the surface-enhanced Raman scattering (SERS) signals in a single-step interaction with CT, because of their assembly into aggregates in response to the CT-sandwiched interactions. The results reveal that the developed nanobeacon provides a simple but ultrasensitive sensor for rapid detection of CT with a large signal-to-background ratio and excellent reproducibility in a wide dynamic range, implying its potential for point-of-care applications in preventive and diagnostic monitoring of cholera.
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| http://dx.doi.org/10.1021/acs.analchem.6b00944 | DOI Listing |
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