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Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. | LitMetric

Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts.

Bio Protoc

Department of Urology, University of California, San Francisco, USA, and Gladstone Institute of Virology and Immunology, San Francisco, USA.

Published: October 2015

AI Article Synopsis

Article Abstract

Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918807PMC
http://dx.doi.org/10.21769/bioprotoc.1623DOI Listing

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