The Genea091 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 40 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 92% of cells expressed Nanog, 97% Oct4, 79% Tra1-60 and 98% SSEA4 and gave a Pluritest pluripotency score of 38.36, Novelty of 1.35. The cell line was negative for Mycoplasma and visible contamination.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.scr.2016.02.022 | DOI Listing |
Publication Analysis
Top Keywords
huntington disease
8
genea091 human
8
human embryonic
8
embryonic stem
8
stem cell
8
derivation huntington
4
disease genea091
4
cell
4
cell genea091
4
cell derived
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!