AI Article Synopsis
- This study aims to explore how microRNA-382 (miR-382) affects the biological functions of human umbilical cord-derived mesenchymal stem cells (hUC-MSC).
- Researchers used specific methods like transfection, microscopy, and staining to assess changes in cell shape, growth, and differentiation into fat and bone cells.
- The findings revealed that while miR-382 doesn't change the cell's shape, growth, or fat cell formation, it does inhibit bone formation by reducing Runx2 expression and influencing key cytokines secreted by the cells.
Article Abstract
Objective: To investigate the effect of microRNA-382 (miR-382) on the biological properties of human umbilical cord-derived mesenchymal stem cells (hUC-MSC).
Methods: The mimics and inhibitor of miR-382 were transfected into hUC-MSC with lipo2000. Inverted microscopy was used to observe the morphology change of hUC-MSC. The proliferation of hUC-MSC was detected by CCK-8. Oil red O and alizarin red staining were applied to assess the adipogenic and osteogenic differentiation of hUC-MSC. Cetylpyridinium chloride was used to the quantitative analysis of osteogenic differentiation. The expression of Runx2 and some cytokines were detected by RT-PCR.
Results: miR-382 did not influence the morphology, proliferation and adipogenic differentiation of hUC-MSC miR-382 inhibited the expression of Runx2, thus could inhibit the osteogenesis of hUC-MSC, being confirmed by alizarin red stain; miR-382 could influence the expression of key cytokines secreted from hUC-MSC, such as IL-6, IDO1, G-CSF, M-CSF, GM-CSF.
Conclusion: miR-382 decreases the expression of Runx2 and inhibites the osteogenesis of hUC-MSC. In addition, it also affects the expression of some key cytokines secreted from hUC-MSC.
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| http://dx.doi.org/10.7534/j.issn.1009-2137.2016.03.040 | DOI Listing |
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