Objective: To explore an efficient, stable system and method to verify the regulation effect of small molecule compounds on human hematopoietic stem cells (hHSC).
Methods: By using combination of flow cytometry with study results of surface markers on hHSC, and optimation of sorting process for further studying the effect of small molecular compounds on stem property of hHSC, the single hHSC was treated with published small molecular compounds such as SR1 and UM171 which possess the expansion effect. After treating with hHSC for 14 d, the flow cytometric analysis of cell phenotypes and cell morphologic observation were performed, at the same time the hematopoietic function of cultured hHSC was verified by colony-forming cell (CFC) test and cobblestone area forming cell (CAFC) test.
Results: The effects of SR1 and UM171 and their compositions in multi-cell culture were consistent with the published data, therefore the useful concentration of compounds were obtained. The results of multiparameter sorting of single cell (CD34+ CD38- CD45RA- CD90+ CD49f+) and ex vivo culture were consistent with the results of bulk cell culture. The results of cell phenotype analysis was in accordance with flow cytometric results. In addition, CFC test and CAFC test revealed that the colony-forming ability in treated group was significantly higher than that in control group (P<0.05).
Conclusion: The rapid, efficient stably amplified and short-time culture system for single hHSC and method for varifying the effect of small molecular compounds are established, which provides platform for screening small molecular compounds and lays the foundation for further study of hHSC expansion.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.7534/j.issn.1009-2137.2016.03.039 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mass Spectrometry Analysis of Chemically and Collisionally Dissociated Molecular Glue- and PROTAC-Mediated Protein Complexes Informs on Disassembly Pathways.
J Am Soc Mass Spectrom
January 2025
Technical University of Darmstadt, Clemens-Schöpf Institute of Organic Chemistry and Biochemistry, Department of Chemistry, Peter-Grünberg-Straße 4, 64287 Darmstadt, Germany.
Molecular glues (MGs) and proteolysis-targeting chimeras (PROTACs) are used to modulate protein-protein interactions (PPIs), via induced proximity between compounds that have little or no affinity for each other naturally. They promote either reversible inhibition or selective degradation of a target protein, including ones deemed undruggable by traditional therapeutics. Though native MS (nMS) is capable of analyzing multiprotein complexes, the behavior of these artificially induced compounds in the gas phase is still not fully understood, and the number of publications over the past few years is still rather limited.
View Article and Find Full Text PDFAtomically Dispersed Fe-Mo Catalysts Mediate Fenton-Like Reaction to Efficiently Degrade Chlorophenol Pollutants Through Synergistic Oxidation and Dechlorination Reactions.
Small
January 2025
School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai, 200093, P. R. China.
Chlorophenols are difficult to degrade and mineralize by traditional advanced oxidation processes due to the strong electronegativity of chlorine. Here, a dual-site atomically dispersed catalyst (FeMoNC) is reported, which Fe/Mo supported on mesoporous nitrogen-doped carbon is prepared through high-temperature migration. The FeMoNC exhibits a high dechlorination rate of 93.
View Article and Find Full Text PDFDonor-Interacting Arylated Carbazole Self-Assembled Monolayer Enables Highly Efficient and Stable Organic Photovoltaics.
Small
January 2025
Department of Materials Science and Engineering, City University of Hong Kong, Kowloon, Hong Kong, 999077, China.
Carbazole-derived self-assembled monolayers (SAMs) are promising materials for hole-extraction layer (HEL) in conventional organic photovoltaics (OPVs). Here, a SAM Cbz-2Ph derived from 3,6-diphenylcarbazole is demonstrated. The large molecular dipole moment of Cbz-2Ph allows the modulation of electrode work function to facilitate hole extraction and maximize photovoltage, thus improving the OPV performance.
View Article and Find Full Text PDFDroplet-Based EPR Spectroscopy for Real-Time Monitoring of Liquid-Phase Catalytic Reactions.
Small Methods
January 2025
Institute of Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir-Prelog-Weg 1, Zürich, 8093, Switzerland.
In situ monitoring is essential for catalytic process design, offering real-time insights into active structures and reactive intermediates. Electron paramagnetic resonance (EPR) spectroscopy excels at probing geometric and electronic properties of paramagnetic species during reactions. Yet, state-of-the-art liquid-phase EPR methods, like flat cells, require custom resonators, consume large amounts of reagents, and are unsuited for tracking initial kinetics or use with solid catalysts.
View Article and Find Full Text PDFMicrobial amidases: Characterization, advances and biotechnological applications.
Biotechnol Notes
December 2024
Centre for Molecular Biology, Central University of Jammu, Rahya Suchani (Bagla), Jammu & Kashmir, India.
The amidases (EC 3.5.1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!