AI Article Synopsis
- BacMam is a recombinant baculovirus that can deliver genes into mammalian cells through endocytosis, with expression levels varying by cell line and cell condition.
- The study found that activating the DNA damage response (DDR) can significantly boost transgene expression up to 12-fold in specific cancer cells, comparable to histone deacetylase inhibitors.
- Combining a Topoisomerase II inhibitor (VM-26) with a BacMam vector enhances both gene expression and cell killing efficiency in osteosarcoma cells, suggesting new therapeutic applications for BacMam.
Article Abstract
BacMam is an insect-derived recombinant baculovirus that can deliver genes into mammalian cells. BacMam vectors carrying target genes are able to enter a variety of cell lines by endocytosis, but the level of expression of the transgene depends on the cell line and the state of the transduced cells. In this study, we demonstrated that the DNA damage response (DDR) could act as an alternative pathway to boost the transgene(s) expression by BacMam and be comparable to the inhibitors of histone deacetylase. Topoisomerase II (Top II) inhibitor-induced DDR can enhance the CMV-IE/enhancer mediated gene expression up to 12-fold in BacMam-transduced U-2OS cells. The combination of a Top II inhibitor, VM-26, can also augment the killing efficiency of a p53-expressing BacMam vector in U-2OS osteosarcoma cells. These results open a new avenue to facilitate the application of BacMam for gene delivery and therapy.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926464 | PMC |
| http://dx.doi.org/10.3390/ijms17060931 | DOI Listing |
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