AI Article Synopsis
- * Three fractions were created using ultrafiltration, with RF30 displaying the strongest effect, reducing paw swelling by 57% at a dosage of 4.9mg/kg.
- * The RF30 polysaccharide has a molecular weight of 91,000g/mol and consists of various sugars, primarily structured as a branched type I rhamnogalacturonan with side chains of arabinogalactans and arabinan.
Article Abstract
The plant Arctium lappa L. is popularly used to relieve symptoms of inflammatory disorders. A crude polysaccharide fraction (SAA) resulting of aqueous extraction of A. lappa leaves showed a dose dependent anti-edematogenic activity on carrageenan-induced paw edema, which persisted for up to 48h. Sequential fractionation by ultrafiltration at 50kDa and 30kDa cut-off membranes yielded three fractions, namely RF50, RF30, and EF30. All these maintained the anti-edematogenic effect, but RF30 showed a more potent action, inhibiting 57% of the paw edema at a dose of 4.9mg/kg. The polysaccharide RF30 contained galacturonic acid, galactose, arabinose, rhamnose, glucose, and mannose in a 7:4:2:1:2:1 ratio and had a Mw of 91,000g/mol. Methylation analysis and NMR spectroscopy indicated that RF30 is mainly constituted by a type I rhamnogalacturonan branched by side chains of types I and II arabinogalactans, and arabinan.
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| http://dx.doi.org/10.1016/j.ijbiomac.2016.06.033 | DOI Listing |
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