The members of the rhodopsin family of proteins are involved in many essential light-dependent processes in biology. Specific photoisomerization of the protein-bound retinylidene PSB at a specified wavelength range of light is at the heart of all of these systems. Nonetheless, it has been difficult to reproduce in an engineered system. We have developed rhodopsin mimics, using intracellular lipid binding protein family members as scaffolds, to study fundamental aspects of protein/chromophore interactions. Herein we describe a system that specifically isomerizes the retinylidene protonated Schiff base both thermally and photochemically. This isomerization has been characterized at atomic resolution by quantitatively interconverting the isomers in the crystal both thermally and photochemically. This event is accompanied by a large pKa change of the imine similar to the pKa changes observed in bacteriorhodopsin and visual opsins during isomerization.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780486 | PMC |
| http://dx.doi.org/10.1021/jacs.6b03681 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
It is now possible to generate large volumes of high-quality images of biomolecules at near-atomic resolution and in near-native states using cryogenic electron microscopy/electron tomography (Cryo-EM/ET). However, the precise annotation of structures like filaments and membranes remains a major barrier towards applying these methods in high-throughput. To address this, we present TARDIS ( ransformer-b sed apid imensionless nstance egmentation), a machine-learning framework for fast and accurate annotation of micrographs and tomograms.
View Article and Find Full Text PDFUnveiling the Dissociation Mechanism of Diglycine Perchlorate.
Inorg Chem
January 2025
High Pressure & Synchrotron Radiation Physics Division, Bhabha Atomic Research Centre, Mumbai, Trombay 400085, India.
Determining the dissociation mechanism of perchlorate materials remains a top priority to address sustainability, handling, processing, and synthesis issues of new and existing high-energy density materials vital to many industrial processes. We determined the dissociation mechanism of diglycine perchlorate (DGPCl) using vibrational spectroscopy, which unveiled the formation of ammonium perchlorate (AP) and carbon at high temperatures. Our studies establish that DGPCl shows multiple phase transitions upon heating.
View Article and Find Full Text PDFEdge-Energy-Driven Growth of Monolayer MnI Islands on Ag(111): High-Resolution Imaging and Theoretical Analysis.
ACS Nano
January 2025
Institute of Physics and Astronomy, University of Potsdam, 14476 Potsdam-Golm, Germany.
The reduced dimensionality of thin transition metal dihalide films on single-crystal surfaces unlocks a diverse range of magnetic and electronic properties. However, achieving stoichiometric monolayer islands requires precise control over the growth conditions. In this study, we employ scanning probe microscopy to investigate the growth of MnI on Ag(111) via single-crucible evaporation.
View Article and Find Full Text PDFReinvestigating atomic ordering in KNaNbOand its impact on ferroelectric properties.
J Phys Condens Matter
January 2025
Physics, Indian Institute of Technology Banaras Hindu University, Indian Institute of Technology (Banaras Hindu University), Department of Physics, Varanasi, Varanasi, Uttar Pradesh, 221005, INDIA.
In the present work, we reinvestigate the atomic ordering of a Pb-free Morphotropic Phase Boundary (MPB) composition viz., K0.5Na0.
View Article and Find Full Text PDFCoordination Equilibrium-Assisted Coprecipitation Synthesis of Atomically Dispersed 3d Metal Catalysts.
ACS Appl Mater Interfaces
January 2025
School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, People's Republic of China.
As a frontier of heterogeneous catalysis, single-atom catalysts (SACs) have been extensively studied fundamentally. One obstacle that limits the industrial application of SACs is the lack of a synthetic method that can prepare the catalysts on a large scale. Wet-chemistry methods that are conventionally used to prepare nanoparticle-based industrial catalysts might be a solution.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!