Biological specimens for microbiological analysis are often collected in BD Vacutainers®, which are not specifically designed for microbial recovery. Bacterial and fungal recovery was analyzed for glass and plastic tubes with or without clot-activating silica. No significant impact was found for the recovery of most bacteria and yeasts tested, however, Haemophilus influenzae recovery from cerebrospinal fluid was significantly reduced in both glass and plastic clot activator tubes.
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http://dx.doi.org/10.1016/j.diagmicrobio.2016.05.015 | DOI Listing |
J Environ Manage
January 2025
Engineering Department, University of Palermo, Viale delle Scienze, Ed. 8, 90128, Palermo, Italy.
The products of an advanced sewage sludge fermentation process can be used to generate polyhydroxyalkanoates (PHAs), precursors of bioplastics considered excellent candidates for replacing petroleum-derived plastics. The aerobic feast-anoxic famine cycling strategy has proven to be an efficient method for enriching sewage sludge microbiota with PHA-producing microorganisms. This work evaluated the effect of different carbon to nitrogen ratios (C/N) of 3.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
College of Water Sciences, Beijing Normal University, Beijing 100875, PR China; Engineering Research Center of Groundwater Pollution Control and Remediation, Ministry of Education, Beijing Normal University, Beijing 100875, PR China. Electronic address:
The limited understanding of microbial response mechanism remains as a bottleneck to evaluate the long-term remediation effectiveness of in situ chemical oxidation in contaminated groundwater. In this study, we investigated long-term response of bacterial communities throughout five remediation stages of pre-oxidation, early-oxidation, late-oxidation, early-recovery and late-recovery. By analyzing bacterial biomass, taxa, diversity and metabolic functions, this work identified the consistently suppressed glyceraldehyde-3-phosphate dehydrogenase pathway and the enrichment of naphthalene degradation pathways for secondary products, suggesting persistent oxidation stress and enhanced microbial utilization of lower-molecular weight carbon sources at the oxidation and early-recovery stages.
View Article and Find Full Text PDFACS Sustain Resour Manag
January 2025
Department of Agrobiotechnology, IFA-Tulln, Institute of Environmental Biotechnology, BOKU University, Vienna, Konrad-Lorenz-Strasse 20, 3430 Tulln an der Donau, Austria.
Tremendous quantities of textile waste generated and primarily landfilled annually represent a huge risk of contaminating the environment, together with loss of valuable resources. Especially, blended fabrics further pose a challenge for recycling and valorization strategies, while enzymatic hydrolysis offers a highly specific and environmentally friendly solution. In this study, we demonstrate that proteases specifically hydrolyze the wool components in blends with polyester, allowing recovery of pure polyester fibers as well as amino acids and peptides as platform molecules for further valorization.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
Key Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, Fujian Normal University, Fuzhou, Fujian 350117, China. Electronic address:
Hyperaccumulators harbor potentials for remediating rare earth elements (REEs)-contaminated soils. However, how they thrive in low-nutrient abandoned REEs mining sites is poorly understood. Three ferns (REEs-hyperaccumulators Dicranopteris pedata and Blechnum orientale, and non-hyperaccumulator Pteris vittata) along with their rhizosphere soils were collected to answer this question by comparing differences in soil nutrient levels, soil and plant REEs concentrations, and bacterial diversity, composition, and functions.
View Article and Find Full Text PDFJ Hazard Mater
January 2025
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, PR China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, PR China. Electronic address:
Copper (Cu)-containing wastewater has proven difficult to effectively treat using the anammox process. In this study, the nitrogen removal efficiency (NRE), sludge characteristics, microbial community and recovery mechanisms of biochar-mediated anammox under Cu stress were elucidated. At a Custress of 5 mg/L, a 73.
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