Flow cytometric enumeration of bacterial in the coral surface mucus layer.

J Microbiol Methods

UMR MARBEC (IRD, CNRS, UM, IFREMER) Marine Biodiversity, Exploitation and Conservation, Montpellier, France.

Published: September 2016

The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.

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http://dx.doi.org/10.1016/j.mimet.2016.05.032DOI Listing

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