Background: Alternative medicine is noted for its clinical effect and minimal invasiveness in the treatment of neuropathic pain. Go-sha-jinki-Gan, a traditional Japanese herbal medicine, has been used for meralgia and numbness in elderly patients. However, the exact mechanism of GJG is unclear. This study aimed to investigate the molecular mechanism of the analgesic effect of GJG in a chronic constriction injury model.
Results: GJG significantly reduced allodynia and hyperalgesia from the early phase (von Frey test, p<0.0001; cold-plate test, p<0.0001; hot-plate test p¼0.011; two-way repeated measures ANOVA). Immunohistochemistry and Western blot analysis revealed that GJG decreased the expression of Iba1 and tumor necrosis factor-a in the spinal cord. Double staining immunohistochemistry showed that most of the tumor necrosis factor-a was co-expressed in Iba1-positive cells at day 3 post-operation. GJG decreased the phosphorylation of p38 in the ipsilateral dorsal horn. Moreover, intrathecal injection of tumor necrosis factor-a opposed the anti-allodynic effect of GJG in the cold-plate test.
Conclusions: Our data suggest that GJG ameliorates allodynia in chronic constriction injury model mice via suppression of tumor necrosis factor-a expression derived from activated microglia. GJG is a promising drug for the treatment of neuropathic pain induced by neuro-inflammation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956397 | PMC |
| http://dx.doi.org/10.1177/1744806916656382 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The microcirculation : master in normal pregnancy, puppet in preeclampsia.
Am J Obstet Gynecol
January 2025
Hasselt University, Faculty of Medicine and Life Sciences, Agoralaan, 3590 Diepenbeek, Belgium; Department of Obstetrics and Gynaecology, ZOL Genk, campus St. Jan, Schiepse Bos 6, 3600 Genk, Belgium.
Objective: To investigate the association between functional parameters of the microcirculation and the systemic cardiovascular system in a population of pregnant women at risk for gestational hypertension disorders.
Study Design: For this observational study, women at high cardiovascular risk according to maternal anthropometrics, obstetric and medical history, were recruited at random gestational age depending on time of referral to the outpatient clinic for high risk prenatal care at Ziekenhuis Oost Limburg, Genk Belgium. After birth, data of maternal and neonatal outcome were obtained from the hospital records: only women with normal pregnancy (n = 142) and with preeclampsia (n = 34) were included in this analysis.
Chronic enteropathy associated with SLCO2A1 from North India: a rare monogenic condition mimicking Crohn's disease.
BMJ Case Rep
January 2025
Pediatrics, All India Institute of Medical Sciences, New Delhi, Delhi, India.
Multiple chronic ulcers of the small intestine are primarily attributed to Crohn's disease. Other differential diagnoses include rare monogenic disorders caused by mutations in and , the latter responsible for chronic enteropathy associated with (CEAS), a condition mainly reported in Asian patients. We present the case of a 10-year-old girl from India with a 5-year history of abdominal pain, altered bowel habits and failure to gain weight.
View Article and Find Full Text PDFElectroacupuncture effects on trigeminal neuralgia with comorbid anxiety and depression: The role of frequency and acupoint specificity.
FASEB J
January 2025
Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
This study aimed to investigate the effects of electroacupuncture (EA) at specific acupoints (DU20 and ST36) and different frequencies (2 and 100 Hz) on brain regions associated with trigeminal neuralgia, anxiety, and depression. Chronic trigeminal neuralgia was induced by the chronic constriction of the infraorbital nerve (CION). Anxiety and depression were assessed through behavioral tests.
View Article and Find Full Text PDF(-) - (11R, 12S)-mefloquine ameliorates neuropathic pain by modulating Cx36-ER stress interaction in the pain-related central nervous system in rats.
Life Sci
January 2025
Key Laboratory of Brain Functional Genomics, Ministry of Education, School of Life Sciences, East China Normal University, Shanghai 200062, China; Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China. Electronic address:
Aims: To explore the specific molecular and cellular mechanisms of (-) - Mefloquine (one of Mefloquine's enantiomers) in modulating the interaction between Connexin 36 (Cx36) and endoplasmic reticulum stress (ERS) both in rats with CCI-induced neuropathic pain and in tunicamycin-induced ERS cells.
Materials And Methods: The authors conducted chronic constriction injury (CCI) in rats to induce neuropathic pain and established the ERS model in SH-SY5Y cells to mimic the stress state after neuropathic pain. The study employed behavioral tests and various molecular biology techniques, including Western blot analysis, cell transfection, and co-immunoprecipitation (co-IP).
Proteomic analysis of spinal dorsal horn in prior exercise protection against neuropathic pain.
Sci Rep
January 2025
Department of Neurobiology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, No. 76 Yanta West Road, Yanta District, Xi'an, 710061, China.
Neuropathic pain (NP) is a complex and prevalent chronic pain condition that affects millions of individuals worldwide. Previous studies have shown that prior exercise protects against NP caused by nerve injury. However, the underlying mechanisms of this protective effect remain to be uncovered.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!