A co-engineered nanoparticle/protein peroxide detector is created. This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP-Gal) that reacts covalently with a boronate-modified green fluorescent protein (PB-GFP). Boronate acid-saccharide complexation between PB-GFP and AuNP-Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101542 | PMC |
| http://dx.doi.org/10.1002/smll.201600986 | DOI Listing |
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