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26-hour Storage of a Declined Liver Before Successful Transplantation Using Ex Vivo Normothermic Perfusion.
Ann Surg
January 2017
Department of Surgery, University of Cambridge, Addenbrooke's Hospital, Cambridge, UK and the NIHR Cambridge Biomedical Research Centre and the NIHR Blood and Transplant Research Unit in Organ Donation and Transplantation at the University of Cambridge, Cambridge, UK.
Delayed processing of blood increases the frequency of activated CD11b+ CD15+ granulocytes which inhibit T cell function.
J Immunol Methods
February 2009
Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15213, United States.
We tested whether granulocytes, which contaminate PBMC isolates after prolonged blood storage at room temperature, are responsible for inhibited T cell function in aged blood. We extend previous observations by characterizing these contaminating granulocytes as CD11b+ CD15+ cells comparable to activated CD11b+ CD15+ granulocytes induced by incubation of blood with FMLP. Granulocyte contamination of PBMC was observed within 6-8 h after venipuncture and room temperature storage (2.
View Article and Find Full Text PDFBrain death-related energetic failure of the donor heart becomes apparent only during storage and reperfusion: an ex vivo phosphorus-31 magnetic resonance spectroscopy study on the feline heart.
J Heart Lung Transplant
September 2001
Heart Lung Institute, University Medical Center, Utrecht, The Netherlands.
Background And Objective: Recently, we have shown, by using localized in vivo phosphorus-31 magnetic resonance spectroscopy (31P MRS) of the anterior left ventricular wall, that brain death (BD) is not associated with reduced myocardial energy status. In this study, we applied ex vivo 31P MRS of the entire heart to study the effects of BD on the energy status of the feline donor heart following explantation.
Methods: We used cats (6 BD and 6 controls [C]) in a 26-hour protocol.
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