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Quality indicators for urine sample contamination: can squamous epithelial cells and bacteria count be used to identify properly collected samples?
Clin Chem Lab Med
January 2025
117303 Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil.
Objectives: To evaluate urinalysis parameters useful for identifying mixed cultures in urine culture using an automated urinary particle analyzer to assess quality indicators (QIs) for urine sample contamination.
Methods: A retrospective observational cross-sectional study was conducted with 2,527 urine samples from patients of a quaternary hospital in Brazil. Urine samples were processed simultaneously in Sysmex UF-5000 flow cytometry analyzer (urinalysis) and MALDI-TOF (culture).
Utility of digital images captured after 4 h of incubation on a microbiology laboratory automation system in guiding the work-up of subcultures from positive blood cultures.
J Clin Microbiol
December 2024
Clinical Microbiology Service, Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
Unlabelled: Initial workup [e.g., identification (ID) and/or antimicrobial susceptibility testing (AST)] of bacterial growth on solid media traditionally occurs 16-24 h after sub-culturing of positive blood cultures (BC).
View Article and Find Full Text PDFUsefulness of differential somatic cell count for udder health monitoring: effect of intramammary infections, days in milk, quarter location, and parity on quarter-level differential somatic cell count and somatic cell score in apparently healthy dairy cows.
J Dairy Sci
December 2024
Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, Canada; Regroupement FRQNT Op+lait, Saint-Hyacinthe, QC, Canada. Electronic address:
Microbial infections of the mammary gland often cause mastitis, and it can lead to substantial economic losses within the dairy industry due to its direct negative impact on milk production and composition and the associated treatment costs. Somatic cell count has emerged as a critical indicator in monitoring udder health, and recently, the large-scale availability of differential cell count analysis potentially offers new insights into underlying physiological processes. Therefore, the main objective of this study was to estimate the variation of DSCC and SCC of individual quarter-level milk samples of cows according to 1) their intramammary infectious status; 2) parity of the cow; 3) quarter location; and 4) DIM at the time of sampling.
View Article and Find Full Text PDFComparison of the molecular FluoroType Mycobacteria VER 1.0 and the Maldi BioTyper Mycobacteria assays for the identification of non-tuberculous mycobacteria.
J Clin Microbiol
December 2024
Laboratoire de Bactériologie, CHU Bordeaux, Bordeaux, France.
Accurate identification of non-tuberculous mycobacterial (NTM) species is crucial for the diagnosis and appropriate management of NTM infections. This study aimed to evaluate the performance of two assays, FluoroType Mycobacteria VER 1.0 and Maldi BioTyper (MBT) Mycobacteria.
View Article and Find Full Text PDFDNA and hemoglobin binding activities: Investigation of coumarin-thiosemicarbazone hybrids.
Bioorg Chem
December 2024
Marmara University, Department of Chemistry, 34722 Istanbul, Turkiye. Electronic address:
Coumarin and coumarin-thiosemicarbazone hybrids were synthesized and characterized by various techniques such as FT-IR, H NMR, C NMR, MALDI-TOF-MS spectroscopy, and single crystal X-Ray diffractometer (XRD). The photochemical and photophysical properties of the compounds, such as solvatochromism, solubility, and chemical reactivity, were analyzed using UV-vis spectroscopy in different solvents. Due to the potential biological activities of the synthesized compounds, their binding affinity and mechanisms with calf thymus DNA (ct-DNA) and bovine hemoglobin (BHb) were determined using several useful spectrophotometric and theoretical approaches such as UV-vis absorption and fluorescence spectroscopy, molecular docking, and density functional theory (DFT).
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