AI Article Synopsis
- Polycomb group proteins play a crucial role in gene repression in higher eukaryotes, but their recruitment mechanism remains unclear.
- The protein LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) interacts primarily with PRC2 to promote the modification H3K27me3, rather than with PRC1 for monoubiquitination.
- The transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 assist in establishing this modification at specific KNOX genes by interacting directly with LHP1, suggesting that they may share additional target genes involved in leaf development.
Article Abstract
Polycomb group proteins are important repressors of numerous genes in higher eukaryotes. However, the mechanism by which Polycomb group proteins are recruited to specific genes is poorly understood. In Arabidopsis, LIKE HETEROCHROMATIN PROTEIN 1 (LHP1), also known as TERMINAL FLOWER 2, was originally proposed as a subunit of polycomb repressive complex 1 (PRC1) that could bind the tri-methylated lysine 27 of histone H3 (H3K27me3) established by the PRC2. In this work, we show that LHP1 mainly functions with PRC2 to establish H3K27me3, but not with PRC1 to catalyze monoubiquitination at lysine 119 of histone H2A. Our results show that complexes of the transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS and KNAT2 via direct interactions with LHP1. Additionally, our transcriptome analysis indicated that there are probably more common target genes of AS1 and LHP1 besides Class-I KNOX genes during leaf development in Arabidopsis.
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| http://dx.doi.org/10.1111/jipb.12485 | DOI Listing |
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