AI Article Synopsis
- Oxygen uptake rate (OUR) and respiratory quotient (RQ) are important for studying DHA production in Schizochytrium sp. HX-308, especially under conditions with limited dissolved oxygen.
- In experiments with varying aeration rates in a 50L bioreactor, higher OUR was linked to better oxygen supply, resulting in a maximum biomass of 124.5g/L.
- DHA content correlated strongly with RQ values, peaking at 44.85% of total fatty acids when RQ was highest, indicating active metabolic pathways, highlighting the potential of OUR and RQ for real-time monitoring of DHA fermentation processes.
Article Abstract
Oxygen uptake rate (OUR) and respiratory quotient (RQ) are key respiratory parameters for docosahexaenoic acid (DHA) production by Schizochytrium sp. HX-308 under dissolved oxygen limited conditions. To investigate the relationship of OUR and RQ with culture status, three independent cultures with different aeration rates were performed in a 50L bioreactor. OUR was found to be positively correlated with the aeration rate, which reflected the oxygen supply level in each culture. The highest biomass, reaching 124.5g/L, was achieved under the highest OUR. DHA content was found to be highly correlated with the RQ value, and the highest DHA content (44.85% in total fatty acids, w/w) was achieved in the highest RQ level, which implies that the polyketide synthase pathway was more active. OUR and RQ, which reflect the physiological state of microorganisms, are suggested as synergistic real-time bioprocess monitoring parameters for DHA fermentation.
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| http://dx.doi.org/10.1016/j.biortech.2016.05.044 | DOI Listing |
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