Effective sampling of biological material is critical to the ability to acquire DNA profiles of probative value. The main methods of collection are swabbing, tapelifting, or direct excision. This chapter describes the key aspects to consider when applying these methods, in addition to suggested procedures for swabbing and tapelifting. Important issues to be considered, such as exhibit triaging, pre-examination preparation, contamination risk reduction, sample localization, sample identification, and sample prioritization as well as aspects of record keeping, packaging, and storage, are also raised.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/978-1-4939-3597-0_1 | DOI Listing |
Forensic Sci Int
December 2020
Office of the Chief Forensic Scientist, Victoria Police Forensic Services Centre, 31 Forensic Drive, Macleod, Victoria, 3085, Australia; School of Molecular Sciences, La Trobe University, Bundoora, Victoria, 3086, Australia.
The ability to recover trace DNA from fired cartridge cases can help establish important leads regarding the handler of the ammunition. Over recent years, several DNA recovery techniques for fired ammunition have been published. Three techniques of significant interest include tape lifting, direct PCR, and vacuum filtration.
View Article and Find Full Text PDFForensic Sci Int Genet
July 2020
College of Science & Engineering, Flinders University, 5042, Adelaide, Australia. Electronic address:
Tape-lifting is a non-destructive alternative to swabbing for collection of biological materials deposited on surfaces, especially on porous substrates. While there have been a number of studies looking at the efficiency of tapes in terms of recovery and their effect on downstream processes, none has been able to visually monitor cellular material collection. We report on a comparative study of a range of tapes regarding their collection efficiency of cellular material visualised using fluorescence microscopy, their background fluorescence after staining with DD diluted with three different solvent types and inhibition of subsequent PCR using direct PCR.
View Article and Find Full Text PDFLeg Med (Tokyo)
November 2017
Institute of Forensic Medicine, Forensic Molecular Biology Dpt., University of Bern, Sulgenauweg 40, 3007 Bern, Switzerland. Electronic address:
To achieve optimal results in the forensic analysis of trace DNA, choosing the right collection technique is crucial. Three common approaches are currently well-established for DNA retrieval from items of clothing, notably cutting, swabbing and tape-lifting. The latter two are non-destructive and therefore preferable on items of value.
View Article and Find Full Text PDFJ Forensic Sci
March 2016
The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA, 22079.
In forensic science, biological material is typically collected from evidence via wet/dry double swabbing with cotton swabs, which is effective but can visibly damage an item's surface. When an item's appearance must be maintained, dry swabbing and tape-lifting may be employed as collection techniques that are visually nondestructive to substrates' surfaces. This study examined the efficacy of alternative swab matrices and adhesive lifters when collecting blood and fingerprints from glass, painted drywall, 100% cotton, and copy paper.
View Article and Find Full Text PDFMethods Mol Biol
January 2018
Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Melbourne, VIC, 3085, Australia.
Effective sampling of biological material is critical to the ability to acquire DNA profiles of probative value. The main methods of collection are swabbing, tapelifting, or direct excision. This chapter describes the key aspects to consider when applying these methods, in addition to suggested procedures for swabbing and tapelifting.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!