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Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation. | LitMetric

Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation.

Curr Protoc Chem Biol

Department of Chemistry and Center for Nucleic Acids Science & Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania.

Published: June 2016

AI Article Synopsis

Article Abstract

Particularly for its use in bioconjugations, the copper-catalyzed (or copper-promoted) azide-alkyne cycloaddition (CuAAC) reaction or 'click chemistry', has become an essential component of the modern chemical biologist's toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocols presented here can be used for either. The reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor co-solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product. © 2016 by John Wiley & Sons, Inc.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940987PMC
http://dx.doi.org/10.1002/cpch.1DOI Listing

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