Genome sequencing and annotation of a Campylobacter coli strain isolated from milk with multidrug resistance.

Genom Data

Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.

Published: June 2016

As the most prevalent bacterial cause of human gastroenteritis, food-borne Campylobacter infections pose a serious threat to public health. Whole Genome Sequencing (WGS) is a tool providing quick and inexpensive approaches for analysis of food-borne pathogen epidemics. Here we report the WGS and annotation of a Campylobacter coli strain, FNW20G12, which was isolated from milk in the United States in 1997 and carries multidrug resistance. The draft genome of FNW20G12 (DDBJ/ENA/GenBank accession number LWIH00000000) contains 1, 855,435 bp (GC content 31.4%) with 1902 annotated coding regions, 48 RNAs and resistance to aminoglycoside, beta-lactams, tetracycline, as well as fluoroquinolones. There are very few genome reports of C. coli from dairy products with multidrug resistance. Here the draft genome of FNW20G12, a C. coli strain isolated from raw milk, is presented to aid in the epidemiology study of C. coli antimicrobial resistance and role in foodborne outbreak.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878843PMC
http://dx.doi.org/10.1016/j.gdata.2016.05.003DOI Listing

Publication Analysis

Top Keywords

coli strain
12
multidrug resistance
12
genome sequencing
8
annotation campylobacter
8
campylobacter coli
8
strain isolated
8
isolated milk
8
resistance draft
8
draft genome
8
genome fnw20g12
8

Similar Publications

Growth inhibition by ppc deletion is rescued by isocitrate dehydrogenase mutations in Escherichia coli.

FEMS Microbiol Lett

January 2025

Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan.

Phosphoenolpyruvate carboxylase encoded by ppc catalyzes the anaplerotic reaction of oxaloacetate in the TCA cycle in Escherichia coli. Deletion of ppc does not prevent the cells from replenishing oxaloacetate via the glyoxylate shunt, but the ppc-deletion strain almost did not grow on glucose. In the present study, we obtained evolved strains by deleting both ppc and mutS to increase the mutation rate and investigated the mechanisms for improving growth by analyzing the mutated genes.

View Article and Find Full Text PDF

Selective pressure of various levels of erythromycin on the development of antibiotic resistance.

Environ Pollut

January 2025

Civil and Construction Engineering and Environmental and Ecological Engineering, Purdue University, 550 Stadium Mall Drive, West Lafayette, Indiana, United States 47907. Electronic address:

This study evaluated microbial fitness under selective pressure of various erythromycin concentrations and the development of resistance genes in Escherichia coli (E. coli) and Enterococcus faecalis (E. faecalis).

View Article and Find Full Text PDF

The cytoplasmic membrane of bacteria is composed of a phospholipid bilayer made up of a diverse set of lipids. Phosphatidylglycerol (PG) is one of the principal constituents and its production is essential for growth in many bacteria. All the enzymes required for PG biogenesis in have been identified and characterized decades ago.

View Article and Find Full Text PDF

A Fast-Pass, Desorption Electrospray Ionization Mass Spectrometry Strategy for Untargeted Metabolic Phenotyping.

J Am Soc Mass Spectrom

January 2025

Department of Chemistry, Center for Innovative Technology, Vanderbilt University, Nashville, Tennessee 37235, United States.

Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) provides direct analytical readouts of small molecules that can be used to characterize the metabolic phenotypes of genetically engineered bacteria. In an effort to accelerate the time frame associated with the screening of mutant libraries, we have developed a high-throughput DESI-MSI analytical workflow implementing a single raster line-scan strategy that facilitates the collection of location-resolved molecular information from engineered strains on a subminute time scale. Evaluation of this "Fast-Pass" DESI-MSI phenotyping workflow on analytical standards demonstrated the capability of acquiring full metabolic profiling information with a throughput of ∼40 s per sample.

View Article and Find Full Text PDF

Post-transcriptional regulation of aromatic amino acid metabolism by GcvB small RNA in .

Microbiol Spectr

January 2025

Department of Infection Biology, Institute of Medicine, University of Tsukuba, Ibaraki, Japan.

synthesizes aromatic amino acids (AAAs) through the common pathway to produce the precursor, chorismate, and the three terminal pathways to convert chorismate into Phe, Tyr, and Trp. also imports exogenous AAAs through five transporters. GcvB small RNA post-transcriptionally regulates more than 50 genes involved in amino acid uptake and biosynthesis in , but the full extent of GcvB regulon is still underestimated.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!