Repetitive formation of optically-observable planar lipid bilayers by rotating chambers on a microaperture.

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Center for International Research on Integrative Biomedical Systems (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba Meguro-ku, Tokyo 153-8505, Japan.

Published: July 2016

Optical observation of a planar lipid bilayer is an effective method of lipid bilayer characterization. However, previous methods for optically observable lipid bilayer formation are unsuitable for repetitive formation of lipid bilayers. In this paper, we propose a system that facilitates repetitive formation of horizontal lipid bilayers via mechanical rotation of the rotating part. We show that multiple bilayers can be observed within a short period, and that the electrical and optical characteristics of a bilayer can be analyzed simultaneously.

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Source
http://dx.doi.org/10.1039/c6lc00363jDOI Listing

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