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Effect of supplemented sericin on the development, cell number, cryosurvival and number of lipid droplets in cultured bovine embryos. | LitMetric

AI Article Synopsis

  • * Although cleavage and blastocyst formation rates were similar across groups, embryos with FBS developed faster, achieving blastulation sooner than those with sericin.
  • * Cryosurvival rates revealed that blastocysts from 0.05% sericin and 5% FBS were more viable after thawing compared to those from higher sericin concentrations, and the quality of 0.05% sericin blastocysts was comparable to those

Article Abstract

Sericin was investigated as an alternative to fetal bovine serum (FBS) for bovine embryo culture. In vitro matured oocytes were developed using 0.05%, 0.1% or 0.15% sericin. The developmental rate, cryosurvival rate and blastulation time of these embryos were compared with those of embryos developed using 5% FBS. The number of lipid droplets was compared among the blastocysts developed using 5% FBS, using 0.05% sericin and in vivo. The rate of cleavage and blastocyst formation was similar among all groups. Blastulation occurred significantly earlier in the embryos developed using 5% FBS than in those developed using sericin at any concentration (P < 0.05). At 72 h after thawing, the cryosurvival rate of the blastocysts developed using 5% FBS and 0.05% sericin were significantly higher compared with those developed using 0.1% and 0.15% sericin (P < 0.05). The blastocysts developed using 0.05% sericin and in vivo produced a significantly fewer number of medium and large lipid droplets than those developed using 5% FBS. These results suggest that the blastocysts developed using 0.05% sericin show characteristics similar to those of the blastocysts developed in vivo and that the use of sericin as an alternative to FBS is feasible.

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Source
http://dx.doi.org/10.1111/asj.12628DOI Listing

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