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Filename: controllers/Detail.php
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File: /var/www/html/application/controllers/Detail.php
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An analysis of Candida albicans ABC transporters identified conserved related α-helical sequence motifs immediately C-terminal of each Walker A sequence. Despite the occurrence of these motifs in ABC subfamilies of other yeasts and higher eukaryotes, their roles in protein function remained unexplored. In this study we have examined the functional significance of these motifs in the C. albicans PDR transporter Cdr1p. The motifs present in NBD1 and NBD2 were subjected to alanine scanning mutagenesis, deletion, or replacement of an entire motif. Systematic replacement of individual motif residues with alanine did not affect the function of Cdr1p but deletion of the M1-motif in NBD1 (M1-Del) resulted in Cdr1p being trapped within the endoplasmic reticulum. In contrast, deletion of the M2-motif in NBD2 (M2-Del) yielded a non-functional protein with normal plasma membrane localization. Replacement of the motif in M1-Del with six alanines (M1-Ala) significantly improved localization of the protein and partially restored function. Conversely, replacement of the motif in M2-Del with six alanines (M2-Ala) did not reverse the phenotype and susceptibility to antifungal substrates of Cdr1p was unchanged. Together, the M1 and M2 motifs contribute to the functional asymmetry of NBDs and are important for maturation of Cdr1p and ATP catalysis, respectively.
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http://dx.doi.org/10.1038/srep27132 | DOI Listing |
Appl Microbiol Biotechnol
December 2024
Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
In the present investigation, 13% ± 0.84 of the extracted and purified phycocyanin from Phormidium versicolor was obtained, with a purity of 0.69 following dialysis.
View Article and Find Full Text PDFInt J Med Mushrooms
December 2024
Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand; Microbiology and Applied Microbiology Research Unit, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham, Thailand.
Candida albicans has the potential to turn pathogenic and cause mild to severe infections, particularly in people with weakened immune systems. Novel therapeutics are required due to its morphological alterations, biofilm development, and resistance to antifungal drugs. Polycephalomyces nipponicus, a traditional East Asian medicinal fungus, has shown potential as an antifungal agent.
View Article and Find Full Text PDFFront Cell Infect Microbiol
December 2024
College of Integrated Chinese and Western Medicine (College of Life Science), Anhui University of Chinese Medicine, Hefei, China.
Among the diverse fungal opportunistic pathogens, garners significant attention due to its wide range of infections and high frequency of occurrence. The emergence of resistance and the limited number of antifungals drives the need to develop novel antifungal drugs. Although the natural product baicalin has been shown to trigger apoptosis in in previous experiments, its influence on cell wall (CW) structure along with immune recognition remains elusive.
View Article and Find Full Text PDFClin Chim Acta
December 2024
Department of Clinical Laboratory, the First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China; Department of Clinical Laboratory, Anhui Public Health Clinical Center, Hefei, People's Republic of China. Electronic address:
Since Candida albicans, a type of fungus, causes severe infections that pose a significant threat to human health, its rapid detection is critical in clinical antifungal therapy. Traditional fungal diagnostic approaches are largely based on the culture method. This method is time-consuming and laborious, taking about 48-72 h, and cannot identify emerging species, making it unsuitable for critically ill patients with bloodstream infections, sepsis, and so on.
View Article and Find Full Text PDFLett Appl Microbiol
December 2024
Graduate School of Science, Osaka Metropolitan University, Osaka 558-8585, Japan.
Thiamine dilauryl sulphate (TDS) exerts a bacteriostatic effect against Gram-positive bacteria and is used as a food additive. The heartwood extract of the Indian kino tree (Pterocarpus marsupium), a medicinal tree native to India, shows weak antimicrobial effects against bacteria and superficial dermatophyte-infective fungi. Herein, we report that the combination of TDS and P.
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