Cultured tissues and cells are used extensively in physiological and pharmacological studies. In vitro cultures provide a means of examining cells and tissues without the complex interactions that would be present if the whole organism were studied. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/cpph.1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Neural correlates of basketball proficiency: An MRI study across skill levels.
J Exerc Sci Fit
January 2025
Center for Studies of Psychological Application, South China Normal University, Guangzhou, 510631, People's Republic of China.
Background: Basketball is an attractive sport required both cooperative and antagonistic motor skills. However, the neural mechanism of basketball proficiency remains unclear. This study aimed to examine the brain functional and structural substrates underlying varying levels of basketball capacity.
View Article and Find Full Text PDFLinks between self-regulation patterns and prosocial behavior trajectories from middle childhood to early adolescence: a longitudinal study.
Front Psychol
December 2024
Department of Psychology, University of Potsdam, Potsdam, Germany.
Prosocial behavior that conforms to social norms and serves the good of others requires particularly high self-regulatory competences, because it is often in contrast with one's own interests. It is unknown which self-regulatory competences are particularly important for prosocial-behavior development and whether they may distinguish between children on different prosocial-behavior trajectories. This longitudinal study examined differences in self-regulatory competences, including inhibition, emotional reactivity, planning behavior, emotion regulation, working-memory updating, affective decision making, flexibility, and delay of gratification, between trajectories of prosocial behavior in 1,657 German 6- to 13-year-olds (52% female).
View Article and Find Full Text PDFMedical students' perceptions of improving physician satisfaction and patient care: a text network analysis approach.
BMC Med Educ
December 2024
Center for Chung-Ang Medical Education Resource Allocation (CAMERA), College of Medicine, Chung-Ang University, Dongjak-gu, Seoul, Republic of Korea.
Background: Physicians' job satisfaction and their relationships with patients are critical factors in modern healthcare. Understanding medical students' perceptions of these aspects is crucial for enhancing the quality of healthcare. Hence, this study examined medical students' perspectives on improving physician job satisfaction and the essential patient-oriented attitudes required for developing into skilled physicians.
View Article and Find Full Text PDFFacilitators' experiences with virtual simulation and their impact on learning.
Adv Simul (Lond)
December 2024
School of Community and Health Studies, Centennial College, P.O. Box 631 Station A, Toronto, ON, Canada.
Background: Increasingly, virtual simulations are being integrated into higher education. A successful experience goes far beyond simply offering learners access to a virtual simulation; it requires a facilitator who understands the learners' needs and course objectives, choses the right virtual simulation for the learner, creates a welcoming space that promotes learning, and evaluates the experience.
Methods: Facilitators from three different healthcare programs and six educational institutions and students from two different healthcare programs were included in this exploratory qualitative research study.
Comparison of the effects of different teaching methods on the effectiveness of teaching neurology in China: a bayesian network meta-analysis and systematic review.
BMC Med Educ
December 2024
Department of Neurology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, 100029, China.
Background: In China, investigations into the efficacy of neurological clinical teaching try to ascertain the impacts of various teaching methods on intervention outcomes. However, these studies often suffer from limited sample sizes, single-center studies and low quality, compounded by the lack of direct comparative analyses between teaching methods, thereby leaving the identification of the most effective method unresolved. This study aims to compare the effectiveness of various teaching methods in the standardized training of Chinese neurology clinicians to inform an optimal teaching model utilizing a Bayesian network meta-analysis (NMA) approach.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!