Pathogen-inducible promoters have been studied extensively and widely used in resistance breeding and gene therapy. However, few reports have been published that explore the efficacy of Bombyx mori nucleopolyhedrovirus (BmNPV)-inducible promoters in antiviral research in the Bombyx mori (Lepidoptera). Here, we screened BmNPV promoters (VP1054, P33, Bm21, Bm122, 39K, P143, and P6.9) and found that the 39K promoter had the highest BmNPV-induced transcriptional activity by dual-luciferase reporter assays system. By 5' truncation analysis, two regions of 39K promoter were critical for optimal virus-inducible activity, indicated that they could serve as a candidate to produce synthetic pathogen-induced promoters. Furthermore, we enhanced the virus-inducible activity of BmNPV 39K promoter using a hybrid enhancer comprising hr3 and polh-up (designated as HP39K). Finally, we showed that RNAi regulated by HP39K promoter could significantly inhibit the proliferation of BmNPV in silkworm cells. Taken together, our results have practical value in antiviral research of silkworm and baculovirus expression system.
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http://dx.doi.org/10.1016/j.jbiotec.2016.05.037 | DOI Listing |
Insect Mol Biol
December 2024
School of Life Sciences, Suzhou Medical College of Soochow University, Suzhou, China.
Starvation can induce autophagy and apoptosis in intestinal cells. To elucidate the underlying mechanisms, we investigated autophagy and apoptosis in the midgut of the model insect, silkworm (Bombyx mori), focusing on calcium homeostasis. The results indicated that the body weight of silkworms decreased, along with damage to the morphology of their digestive tracts and midguts after starvation treatment.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400715, China; Yibin Academy of Southwest University, Southwest University, Chongqing 400715, China. Electronic address:
Endoreplication is particularly important in the context of silk protein synthesis within the silk gland cells of silkworms. Our previous research indicated that the BmE2F1 enhances the silk yield of silkworm cocoons, but the underlying molecular mechanism remains elusive. In this study, we employed RNA-sequencing to dissect the transcriptional profiles of silk glands in the wild-type Dazao silkworm strain and the overexpression (OE) silkworm strain with specific overexpression of the BmE2F1 gene in silk glands.
View Article and Find Full Text PDFZhongguo Zhong Yao Za Zhi
September 2024
Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.
A method for simultaneous determination of multiple mycotoxins in Bombyx Batryticatus and Eupolyphaga Steleophaga was established to provide technical support for monitoring and quality control of such medicinal materials. The mildew-prone Bombyx Batryticatus and Eupolyphaga Steleophaga were detected by UPLC-MS/MS technology, and the chromatographic conditions, mass spectrometry conditions, test sample preparation methods, and QuEChERS purification parameters were optimized. Scanning was conducted under multiple reaction monitoring mode(MRM), and quantitative analysis was performed by matrix standard curve method.
View Article and Find Full Text PDFBMC Res Notes
December 2024
Department of Cell and Molecular Biology, Uppsala University, Box 549, 751 24, Uppsala, Sweden.
Objective: Bombyx mori nucleopolyhedrovirus (BmNPV) causes grasserie with severe effects in Thai strains of the silkworm Bombyx mori. The aim of this study was to investigate the effect of probiotic supplementation on the survival of silkworm larvae challenged with BmNPV.
Results: Silkworm larvae of the Thai polyvoltine strain Samrong was supplemented with commercial probiotic bacteria, Lactobacillus acidophilus, on the second day of the 2nd, 3rd, 4th, and 5th instar.
J Gen Virol
December 2024
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, PR China.
The Bombyx mori nucleopolyhedrovirus (BmNPV) is a DNA virus that affects the silkworm, , causing substantial economic losses in sericulture. This study investigates the mechanisms underlying budded virus egress, focusing on the roles of the ubiquitin-proteasome pathway (UPP) machinery. BmNPV produces two virion types: budded virions (BVs) and occlusion-derived virions (ODVs), which differ in their envelope origins and functions.
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