[This corrects the article DOI: 10.1371/journal.pone.0139253.].
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Nat Commun
March 2024
Department of Chemistry, Hanyang University, Seoul, 04763, Republic of Korea.
PLoS Genet
September 2022
Laboratory of Pharmaceutical Biotechnology, Ghent University, Ghent, Belgium.
CYP2D6 is a very important pharmacogene as it is responsible for the metabolization or bioactivation of 20 to 30% of the clinically used drugs. However, despite its relatively small length of only 4.4 kb, it is one of the most challenging pharmacogenes to genotype due to the high similarity with its neighboring pseudogenes and the frequent occurrence of CYP2D6-CYP2D7 hybrids.
View Article and Find Full Text PDFLancet Microbe
August 2022
Department of Medical Microbiology, University of Amsterdam, Amsterdam UMC, Amsterdam, Netherlands; Department of Global Health, Amsterdam Institute for Global Health and Development (AIGHD), University of Amsterdam, Amsterdam UMC, Amsterdam, Netherlands.
Background: Semi-quantitative bacterial culture is the reference standard to diagnose urinary tract infection, but culture is time-consuming and can be unreliable if patients are receiving antibiotics. Metagenomics could increase diagnostic accuracy and speed by sequencing the microbiota and resistome directly from urine. We aimed to compare metagenomics to culture for semi-quantitative pathogen and resistome detection from urine.
View Article and Find Full Text PDFJ Clin Med
January 2022
Unité Parasitologie et Entomologie, Département Microbiologie et Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, 91220 Marseille, France.
SARS-CoV-2 has caused a large outbreak since its emergence in December 2019. COVID-19 diagnosis became a priority so as to isolate and treat infected individuals in order to break the contamination chain. Currently, the reference test for COVID-19 diagnosis is the molecular detection (RT-qPCR) of the virus from nasopharyngeal swab (NPS) samples.
View Article and Find Full Text PDFMol Ecol Resour
January 2022
Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
Mito-metagenomics (MMG) is becoming an alternative to amplicon metabarcoding for the assessment of biodiversity in complex biological samples using high-throughput sequencing. Whereas MMG overcomes the biases introduced by the PCR step in the generation of amplicons, it is not yet a technique free of shortcomings. First, as the reads are obtained from shotgun sequencing, a very low proportion of reads map into the mitogenomes, so a high sequencing effort is needed.
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