AI Article Synopsis
- The penton-dodecahedron (Pt-Dd), derived from adenovirus type 3, is a virus-like particle with a size smaller than the virus, produced efficiently in a baculovirus system.
- It contains key proteins that can be recognized by various cell surfaces, allowing for effective internalization, as observed through fluorescent microscopy in living HeLa cells.
- The study demonstrates that Pt-Dd can effectively deliver horseradish peroxidase (HRP) into cells without disrupting its enzymatic function, positioning it as a promising alternative for therapeutic delivery systems.
Article Abstract
Penton-dodecahedron (Pt-Dd) derived from adenovirus type 3 is a symmetric complex of pentameric penton base plus fiber which can be produced in the baculovirus system at a high concentration. The size of Pt-Dd is smaller than the virus, but this virus-like particle (VLP) has the major proteins recognized by specific receptors on the surface of almost all types of cell. In this study, by direct observation with fluorescence microscopy on a fixed and living cell, the intracellular trafficking and localization of Pt-Dd labeled with fluorescence dyes in the cytoplasm of HeLa Tub-GFP showed a rapid internalization characteristic. Subsequently, the linkage of horseradish peroxidase (HRP) with Pt-Dd as the vector demonstrated an efficient system to deliver this enzyme into the cell without interfering its enzymatic activity as shown by biochemical and cellular experiments. These results were supported by additional studies using Bs-Dd or free form of the HRP used as the control. Overall, this study strengthens the potential role of Pt-Dd as an alternative vector for delivering therapeutic agents.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4875972 | PMC |
| http://dx.doi.org/10.1155/2016/5030589 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of UHT milk spoilage caused by proteases from psychrophilic bacteria based on peptidomics.
Food Chem X
December 2024
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
The quality issues of ultra-high-temperature (UHT) milk, such as protein hydrolysis and aging gels throughout shelf life, are caused by proteases from psychrophilic bacteria. However, existing enzyme activity detection techniques have low sensitivity and cannot accomplish the detection of product deterioration caused by low enzyme activity. In this study, an attempt was made to analyze the relationship between enzymatically cleaved peptides and product quality using peptidomics techniques.
View Article and Find Full Text PDFDecoding the enigma: unveiling the transmission characteristics of waterfowl-associated -positive in select regions of China.
Front Microbiol
December 2024
Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
() serves as a critical indicator microorganism for assessing the prevalence and dissemination of antibiotic resistance, notably harboring various antibiotic-resistant genes (ARGs). Among these, the emergence of the gene represents a significant threat to public health, especially since carbapenem antibiotics are vital for treating severe infections caused by Gram-negative bacteria. This study aimed to characterize the antibiotic resistance features of -positive strains isolated from waterfowl in several regions of China and elucidate the dissemination patterns of the gene.
View Article and Find Full Text PDFFood utility potential of protease obtained from insect-commensal Bacillus subtilis AU-2.
J Sci Food Agric
December 2024
Microbiology Department, The Institute of Science, Dr. Homi Bhabha State University, Mumbai, India.
Background: Bacillus subtilis AU-2, isolated from the gut of Tribolium castaneum, was used for protease production. The purified protease was evaluated for its potential in food-related applications including meat tenderization, milk coagulation, and the preparation of enzymatic soybean hydrolysates. Enzymatic hydrolysis of soy protein is an effective method for producing protein hydrolysates with optimal techno-functional properties.
View Article and Find Full Text PDFA novel self-assembling peptide nanofiber hydrogel with glucagon-like peptide-1 functionality enhances islet survival to improve islet transplantation outcome in diabetes treatment.
J Nanobiotechnology
December 2024
NHC Key Laboratory for Critical Care Medicine, School of Medicine, Tianjin First Central Hospital, Research Institute of Transplant Medicine, Organ Transplant Center, Nankai University, Tianjin, 300071, China.
Islet transplantation is a promising therapy for diabetes, yet the limited survival and functionality of transplanted islet grafts hinder optimal outcomes. Glucagon-like peptide-1 (GLP-1), an endogenous hormone, has shown potential to enhance islet survival and function; however, its systemic administration can result in poor localization and undesirable side effects. To address these challenges, we developed a novel peptide-based nanofiber hydrogel incorporating GLP-1 functionality for localized delivery.
View Article and Find Full Text PDFA Redox-Enzyme Integrated Microbial Fuel Cell Design Using the Surface Display System in MR-1.
ACS Appl Mater Interfaces
December 2024
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
A biofuel cell is an electrochemical device using exoelectrogen or biocatalysts to transfer electrons from redox reactions to the electrodes. While wild-type microbes and natural enzymes are often employed as exoelectrogen and biocatalysts, genetically engineered or modified organisms have been developed to enhance exoelectrogen activity. Here, we demonstrated a redox-enzyme integrated microbial fuel cell (REI-MFC) design based on an exoelectrogen-enhancing strategy that reinforces the electrogenic activity of MR1 by displaying an extra redox enzyme on the cell surface.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!