Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit.

Methods Mol Biol

Monoclonal Antibody Unit, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore, 138673, Singapore.

Published: December 2017

Monoclonal antibodies offer high specificity and this makes it an important tool for molecular biology, biochemistry and medicine. Typically, monoclonal antibodies are generated by fusing mouse spleen cells that have been immunized with the desired antigen with myeloma cells to create immortalized hybridomas. Here, we describe the generation of monoclonal antibodies that are specific to Chikungunya virus using ClonaCell-HY system.

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Source
http://dx.doi.org/10.1007/978-1-4939-3618-2_20DOI Listing

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