The number of simultaneous liver-kidney transplants has been increasing. This surgery is associated with an increased risk of complications, longer duration of surgery and longer ischemia time for the renal allograft. Two patients listed for liver-kidney transplant at our center underwent en bloc combined liver-kidney transplantation using donor splenic artery as inflow. Patient 1 previously underwent cardiac catheterization that was complicated by a bleeding pseudoaneurysm of the right external iliac artery that required endovascular stenting of the external iliac artery and embolization of the inferior epigastric artery. Patient 2 was on vasopressor support and continuous renal replacement therapy at the time of transplant. In this paper, we described a novel technique of en bloc liver-kidney transplant with simultaneous reperfusion of both allografts using the donor splenic artery for renal inflow. This technique is useful for decreasing cold ischemia time and total operative time by simultaneous reperfusion of both allografts. It is a useful technical variant that can be used in patients with severe disease of the iliac arteries.
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http://dx.doi.org/10.1111/ajt.13885 | DOI Listing |
J Med Life
January 2025
Faculty of Medicine, Hebrew University, Jerusalem, Isreal.
Interactions between immune system constituents are mediated through direct contact or the transfer of mediators. The study aimed to assess the correlation between system components and out-of-body signals in a model of liver inflammation. In the first experiment, mice injected with Concanavalin A (ConA) were housed in a cage with a tube on top containing healthy livers or livers harvested from mice injected with ConA.
View Article and Find Full Text PDFJ Clin Invest
March 2025
INSERM, BIGR, Université Paris Cité, Paris, France.
Although refrigerated storage slows the metabolism of volunteer donor RBCs, which is essential in transfusion medicine, cellular aging still occurs throughout this in vitro process. Storage-induced microerythrocytes (SMEs) are morphologically-altered senescent RBCs that accumulate during storage and are cleared from circulation following transfusion. However, the molecular and cellular alterations that trigger clearance of this RBC subset remain to be identified.
View Article and Find Full Text PDFJ Clin Exp Hepatol
February 2025
Liver Transplant and Biliary Sciences, Gastroenterology, Hepatology & Endoscopy, Max Super Speciality Hospital, Saket, New Delhi, 110017, India.
Purpose: Splenic artery embolization (SAE) is a minimally invasive interventional radiology (IR) procedure that plays an important role in the management of complications in a certain subgroup of liver transplant recipients. SAE is technically of two types - proximal (P-SAE) and distal (D-SAE). There is limited data regarding the role of SAE in liver transplant recipients.
View Article and Find Full Text PDFMethods Mol Biol
March 2025
Institute of Immunology, Ulm University Medical Center, Ulm, Germany.
Germinal center (GC) reaction is crucial for the generation of high-affinity antibodies against any infection. To address the role of specific genes in GC, knockout mouse models are generally used. However, since GC is a multicellular event, complete knockout models cannot pinpoint the cell-intrinsic effect of certain genes in GC reaction.
View Article and Find Full Text PDFMethods Mol Biol
March 2025
Division of Immunology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
B lymphocytes are a critical part of the adaptive immune response elicited by the immune system to fight various pathogens. The main effector function of the B lymphocytes is the ability to secrete antibodies, whether nonclass-switched immunoglobulin M (IgM) or class-switched immunoglobulin isotypes. To understand the function of B cells in vivo, mice are subjected to bone marrow depletion (using radiation or chemical radiation) before being adoptively transferred with donor bone marrow.
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