Recognition of the existence of biofilm in chronic wounds is increasing among wound care practitioners, and a growing body of evidence indicates that biofilm contributes significantly to wound recalcitrance. While clinical guidelines regarding the involvement of biofilm in human bacterial infections have been proposed, there remains uncertainty and lack of guidance towards biofilm presence in wounds. The intention of this report is to collate knowledge and evidence of the visual and indirect clinical indicators of wound biofilm, and propose an algorithm designed to facilitate clinical recognition of biofilm and subsequent wound management practices.
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Adv Sci (Weinh)
January 2025
State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, 2 Southeast University Road, Nanjing, 211189, P. R. China.
Gram-positive bacteria pose significant threats to human health, necessitating the development of targeted bacterial detection and eradication strategies. Nevertheless, current approaches often suffer from poor targeting specificity. Herein, the study utilizes purple rice lixivium to synthesize biomass carbon dots (termed BCDs) with wheat germ agglutinin-like residues for precisely targeting Gram-positive bacteria.
View Article and Find Full Text PDFGels
January 2025
Department of Mechatronics Engineering, Engineering Faculty, Istanbul Ticaret University, 34854 Istanbul, Türkiye.
The aim of this project is to fabricate fiber mats and hydrogel materials that constitute the two main components of a wound dressing material. The contributions of boric acid (BA) and zinc oxide (ZnO) to the physical and mechanical properties of polycaprolactone (PCL) is investigated. These materials are chosen for their antimicrobial and antifungal effects.
View Article and Find Full Text PDFHeliyon
January 2025
Department of Basic Medical Sciences, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran.
Background: This study aimed to evaluate the biofilm formation abilities of clinical strains, assess their antibiotic susceptibility patterns, and identify the prevalence of adhesion-associated genes.
Methodology: In this study, a total of 60 strains were collected from urine, pus, wounds, blood, body fluid, and sputum in health centers affiliated with Abadan University of Medical Sciences, Iran. Strains were identified via microbiological methods and polymerase chain reaction (PCR) to target the gene.
Acta Biomater
January 2025
Department of radiology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214122, China. Electronic address:
Nanohybrids combining phenylboronic acid-modified carbon dots (PCDs) and proteinase K have been engineered for addressing the formidable challenges of antimicrobial photodynamic therapy (aPDT) against bacterial biofilm infections, overcoming biofilm barrier obstruction, the limited diffusion of reactive oxygen species (ROS), and the inadequate ROS generation of traditional photosensitizers. PCDs are formulated for superior water solubility and robust singlet oxygen (O) production, mitigating issues related to dispersion and aggregation-induced quenching typical of conventional photosensitizers. The conjugation of phenylboronic acid to CDs not only enhanced O generation through increased electron-hole separation but also imparted strong bacterial binding capabilities to the PCDs, enabling broad-spectrum sterilization by maximizing the ROS-mediated bacterial destruction.
View Article and Find Full Text PDFAm J Forensic Med Pathol
January 2025
From the Department of Pathology, University of Nevada Reno School of Medicine.
Necrotizing wound infections are potentially lethal complications of surgeries, including cesarean deliveries. A 32-year-old female with obesity and hidradenitis suppurativa (HS) underwent uncomplicated cesarean section. Four days later, she developed abdominal pain and imaging showed ascites; she was treated with antibiotics.
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