AI Article Synopsis
- The study focuses on comparing different Cas9-based transcription factor systems to see how effective they are at activating gene expression in various cell types, including human, mouse, and fly cells.
- Researchers aim to uncover which systems are the most powerful and if combining top activators can enhance gene expression further.
- The investigation also looks into how cooperative interactions between activators contribute to maximizing the level of gene expression achieved.
Article Abstract
Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927356 | PMC |
| http://dx.doi.org/10.1038/nmeth.3871 | DOI Listing |
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