AI Article Synopsis
- The use of transgenic crops has heightened concerns about the presence of Cry proteins in the environment, leading to the adoption of ELISAs for detection.
- ELISA tests may overestimate Cry protein concentrations by detecting fragments that do not maintain their biological activity, raising accuracy questions.
- In a study using five model systems, only fragments from one system were detectable by ELISA and were biologically active, supporting that ELISAs typically provide reliable estimates of Cry protein amounts in environmental samples.
Article Abstract
The continuing use of transgenic crops has led to an increased interest in the fate of insecticidal crystalline (Cry) proteins in the environment. Enzyme-linked immunosorbent assays (ELISAs) have emerged as the preferred detection method for Cry proteins in environmental matrices. Concerns exist that ELISAs are capable of detecting fragments of Cry proteins, which may lead to an overestimation of the concentration of these proteins in the environment. Five model systems were used to generate fragments of the Cry1Ab protein, which were then analyzed by ELISAs and bioassays. Fragments from 4 of the model systems were not detectable by ELISA and did not retain bioactivity. Fragments from the proteinase K model system were detectable by ELISA and retained bioactivity. In most cases, ELISAs appear to provide an accurate estimation of the amount of Cry proteins in the environment, as detectable fragments retained bioactivity and nondetectable fragments did not retain bioactivity. Environ Toxicol Chem 2016;35:3101-3112. © 2016 SETAC.
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