AI Article Synopsis
- Insulin is a hormone that helps regulate blood glucose levels and works with carbohydrates and lipids.
- Researchers developed dextran-encapsulated gold nanoparticles and nanoclusters that can specifically recognize insulin, with a binding strength quantified by a specific dissociation constant.
- The study found that a particular sequence on the B chain of insulin interacts with these nanoprobes, demonstrating their potential to detect low insulin concentrations in serum samples.
Article Abstract
Insulin is a peptide hormone that can regulate the metabolism of carbohydrates and lipids. This hormone is closely related to glucose-uptake in cells and can control blood glucose levels. Dextran is a polysaccharide composed of glucose units. In this study, we discovered that dextran-encapsulated gold nanoparticles (AuNPs@Dextran) and nanoclusters (AuNCs@Dextran) can be used to recognize insulin. The dissociation constant of insulin toward AuNPs@Dextran was estimated to be ∼5.3 × 10 M. The binding site on insulin toward the dextran on the nanoprobes was explored as well. It was found that the sequence of numbers 1-22 on the insulin B chain can interact with the dextran encapsulated nanoprobes. Additionally, we also demonstrated that the dextran-encapsulated nanoprobes could be used as concentration probes to selectively enrich trace amounts of insulin (∼1 pM) from serum samples. Copyright © 2016 John Wiley & Sons, Ltd.
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