Temporal dietary shift in jellyfish revealed by stable isotope analysis.

A temporal change in the stable isotope (SI) composition of jellyfish in the Kiel Fjord, Western Baltic Sea, was documented by analyzing δC, δN and δS of bell tissue of and in the period between June and October 2011. A strong and significant temporal change in all SI values of was found, including an increase of ~3 ‰ in δC, a decrease of ~4 ‰ in δN and sharp decline of ~7 ‰ in δS. While knowledge gaps in jellyfish isotope ecology, in particular the lack of reliable trophic enrichment factors, call for a conservative interpretation of our data, observed changes in particular in δS, as indicated by means of a MixSIR mixing model, would be consistent with a temporal dietary shift in from mesozooplankton (>150 µm) to microplankton and small re-suspended particles (0.8-20 µm) from the benthos. Presence of a hitherto unidentified food source not included in the model could also contribute to the shift. During the 2-month occurrence of , its isotope composition remained stable and was consistent with a mainly mesozooplanktonic diet. Mixing model output, mainly driven by δS values, indicated a lower proportion of in the diet of than previously reported, and thus to a potentially lesser importance of intraguild predation among jellyfish in the Kiel Fjord. Overall, our results clearly highlighted the potential for substantial intraspecific isotopic seasonal variation in jellyfish, which should be taken into account in future feeding ecology studies on this group.