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Inert splint-driven oligonucleotide assembly.
Synth Biol (Oxf)
December 2024
Claret Bioscience LLC, 100 Enterprise Way, Suite A102, Scotts Valley, CA 95066, United States.
In this study, we introduce a new method for oligonucleotide fragment assembly. Unlike polymerase chain assembly and ligase chain assembly that rely on short, highly purified oligonucleotides, our method, named , uses a one-tube, splint-driven assembly reaction. Splynthesis connects standard-desalted "contig" oligos (∼150 nt in length) via shorter "splint" oligos harboring 5' and 3' blocking modifications to prevent off-target ligation and amplification events.
View Article and Find Full Text PDFFor whom the T cells troll? Bispecific T-cell engagers in glioblastoma.
J Immunother Cancer
November 2021
Department of Neurosurgery, Duke University, Durham, North Carolina, USA
Glioblastoma is the the most common primary brain tumor in adults. Onset of disease is followed by a uniformly lethal prognosis and dismal overall survival. While immunotherapies have revolutionized treatment in other difficult-to-treat cancers, these have failed to demonstrate significant clinical benefit in patients with glioblastoma.
View Article and Find Full Text PDFHigh-contrast, synchronous volumetric imaging with selective volume illumination microscopy.
Commun Biol
February 2020
Translational Imaging Center, University of Southern California, Los Angeles, CA, 90089, USA.
Article Synopsis
- - Light-field fluorescence microscopy allows for quick 3D imaging but struggles with low contrast due to background noise from broad illumination techniques.
- - The new method, selective volume illumination microscopy (SVIM), focuses the light only on the area of interest, effectively reducing background and significantly improving image contrast.
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A ligation-based single-stranded library preparation method to analyze cell-free DNA and synthetic oligos.
BMC Genomics
December 2019
Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, 95064, USA.
Background: Cell-free DNA (cfDNA), present in circulating blood plasma, contains information about prenatal health, organ transplant reception, and cancer presence and progression. Originally developed for the genomic analysis of highly degraded ancient DNA, single-stranded DNA (ssDNA) library preparation methods are gaining popularity in the field of cfDNA analysis due to their efficiency and ability to convert short, fragmented DNA into sequencing libraries without altering DNA ends. However, current ssDNA methods are costly and time-consuming.
View Article and Find Full Text PDFReturning to the Patent Landscapes for Nanotechnology: Assessing the Garden that It Has Grown Into.
Methods Mol Biol
February 2018
Center for the Study of Law, Science and Technology, Sandra Day O'Connor College of Law, Arizona State University, 877906, Tempe, AZ, 85297, USA.
The patent landscape, like a garden, can tell you much about its designers and users; their motivations, biases, and general interests. While both patent landscapes and gardens may appear to the casual observer as refined and ordered, an in-depth exploration of the terrain is likely to reveal unforeseen challenges including, for example, alien species, thickets, and trolls. As this Chapter illustrates, patent landscapes are dynamic and have been forced to continually evolve in response to technological innovation.
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