Paramecium bursaria (Ehrenberg 1831), a freshwater ciliate, typically harbors hundreds of green algal symbionts inside the cell. The aim of present study was the molecular identification of newly analyzed P. bursaria symbionts. The second aspect of the present survey was testing a hypothesis whether endosymbionts prefer the specified syngen of the host, and the specified geographical distribution. Ten strains of endosymbionts isolated from strains of P. bursaria originating from different geographical locations were studied. We analyzed for the first time, both the fragment of plastid genome containing 3'rpl36-5' infA genes and a fragment of a nuclear gene encoding large subunit ribosomal RNA (LSU rDNA). The analysis of the LSU rDNA sequences showed the existence of 3 haplotypes and the haplotype diversity of 0.733, and 8 haplotypes for the 3'rpl36-5' infA gene fragment and haplotype diversity of 0.956. The endosymbionts isolated from P. bursaria strains were identified as Chlorella vulgaris, Ch. variabilis and Micractinium conductrix. There was no correlation between the syngen of P. bursaria and the species of endosymbiont.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3409/fb64_1.47 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of the Symbiotic on the Host Ciliate Phenotypes.
Microorganisms
December 2024
Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8512, Yamaguchi, Japan.
, a ciliated protist, forms a symbiotic relationship with the green alga . This endosymbiotic association is a model system for studying the establishment of secondary symbiosis and interactions between the symbiont and its host organisms. Symbiotic algae reside in specialized compartments called perialgal vacuoles (PVs) within the host cytoplasm, which protect them from digestion by host lysosomal fusion.
View Article and Find Full Text PDFRhodotorula mucilaginosa: a new potential human pathogen found in the ciliate Paramecium bursaria.
Protoplasma
January 2025
Core Facility Center "Cultivation of Microorganisms", Saint-Petersburg State University, Saint-Petersburg, Russian Federation.
Ciliates often form symbiotic associations with other microorganisms, both prokaryotic and eukaryotic. We are now starting to rediscover the symbiotic systems recorded before molecular analysis became available. Here, we provide a morphological and molecular characterization of a symbiotic association between the ciliate Paramecium tritobursaria and the yeast Rhodotorula mucilaginosa (syn.
View Article and Find Full Text PDFChromomeres, Topologically Associating Domains and Structural Organization of Chromatin Bodies in Somatic Nuclei (Macronuclei) of Ciliates.
Front Biosci (Landmark Ed)
November 2024
Department of Cancer Cell Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Background: In the twentieth century, the textbook idea of packaging genomic material in the cell nucleus and metaphase chromosomes was the presence of a hierarchy of structural levels of chromatin organization: nucleosomes - nucleosomal fibrils -30 nm fibrils - chromomeres - chromonemata - mitotic chromosomes. Chromomeres were observed in partially decondensed chromosomes and interphase chromatin as ~100 nm globular structures. They were thought to consist of loops of chromatin fibres attached at their bases to a central protein core.
View Article and Find Full Text PDFUnveiling the GT114 family: Structural characterization of A075L, a glycosyltransferase from Paramecium bursaria chlorella virus-1 (PBCV-1).
Protein Sci
December 2024
Basque Research and Technology Alliance (BRTA), Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio, Spain.
Protein A075L is a β-xylosyltransferase that participates in producing the core of the N-glycans found in VP54, the major viral capsid protein of Paramecium bursaria chlorella virus-1 (PBCV-1). In this study, we present an X-ray crystallographic analysis of the apo form of A075L, along with its complexes with the sugar donor and with a trisaccharide acceptor. The protein structure shows a typical GT-B folding, with two Rossmann-like fold domains, in which the acceptor substrate binds to the N-terminal region, and the nucleotide-sugar donor binds to the C-terminal region.
View Article and Find Full Text PDFDynamics of digestive vacuole differentiation clarified by the observation of living Paramecium bursaria.
Protoplasma
October 2024
Institute of Agricultural and Life Sciences, Academic Assembly, Shimane University, Matsue-Shi, Japan.
Paramecium bursaria is a ciliate species that has a symbiotic relationship with Chlorella spp. This study aimed to elucidate the dynamics of digestive vacuole (DV) differentiation in P. bursaria, using yeast stained with a pH indicator.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!