AI Article Synopsis
- Glycosylphosphatidylinositol (GPI) anchoring is a modification that allows proteins to attach to the cell membrane and can be re-inserted into lipid bilayers through a process called Molecular Painting.
- Researchers successfully inserted the protein CD59, which protects cells from the immune system's complement cascade, into retrovirus particles.
- The modified virus showed enhanced infectivity when exposed to human serum, suggesting that Molecular Painting could be beneficial for improving gene therapy and vaccination strategies by altering immune responses.
Article Abstract
Glycosylphosphatidylinositol anchoring is a type of post-translational modification that allows proteins to be presented on the exterior side of the cell membrane. Purified glycosylphosphatidylinositol-anchored protein can spontaneously re-insert into lipid bilayer membranes in a process termed Molecular Painting. Here, we demonstrate the possibility of inserting purified, recombinant CD59 into virus particles produced from a murine retroviral producer cell line. CD59 is a regulator of the complement system that helps protect healthy cells from the lytic activity of the complement cascade. In this study, we could show that Molecular Painting confers protection from complement activity upon murine retroviral vector particles. Indeed, increased infectivity of CD59-modified virus particles was observed upon challenge with human serum, indicating that Molecular Painting is suitable for modulating the immune system in gene therapy or vaccination applications.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4899500 | PMC |
| http://dx.doi.org/10.1007/s12033-016-9944-z | DOI Listing |
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