AI Article Synopsis
- This study examines how ovariectomy (removal of ovaries) affects the plasma concentration of digoxin (DIG) in female rats.
- Twelve rats were split into two groups - one underwent ovariectomy while the other was a control group, and DIG levels were measured after administration.
- Results showed that the highest concentration (C(max)) of DIG was significantly lower in the ovariectomized rats, linked to reduced estrogen and increased levels of P-glycoprotein (P-gp) in their intestines.
Article Abstract
This study aims to investigate the change of plasma concentration of digoxin (DIG) in rats with ovariectomy. Twelve female SD rats were randomly assigned into ovariectomized group and sham group (n = 6). All rats plasma was collected after a single dose of 2 mg x kg(-1) DIG administrated orally, serum DIG concentration was determined by LC-MS/MS. The level of P-gp in the intestinal was analyzed by Western blotting. Pharmacokinetic calculations were performed on each individual using DAS 2.0 practical pharmacokinetic software. Compared with the sham group, C(max) of ovariectomized group decreased significantly (P < 0.01). There was no significant difference of AUC(0-t), and the level of P-gp was elevated in ovariectomized group. It was found that C(max) of DIG was significantly reduced after ovariectomy, and the change was associated with the decreased level of estrogen, which contributes to the increased level of P-gp.
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