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A Highly Efficient System for Separating Glandular and Non-glandular Trichome of Cucumber Fruit for Transcriptomic and Metabolomic Analysis.
Bio Protoc
January 2025
Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing, China.
Cucumber () trichomes play a critical role in resisting external biological and abiotic stresses. Glandular trichomes are particularly significant as they serve as sites for the synthesis and secretion of secondary metabolites, while non-glandular trichomes are pivotal for determining the appearance quality of cucumbers. However, current methods for separating trichomes encounter challenges such as low efficiency and insufficient accuracy, limiting their applicability in multi-omics sequencing studies.
View Article and Find Full Text PDFComparison of diagnostic performance for pulmonary nodule detection between free-breathing spiral ultrashort echo time and free-breathing radial volumetric interpolated breath-hold examination.
BMC Med Imaging
January 2025
School of Medical Technology, Shaanxi University of Chinese Medicine, Xian Yang, 712046, China.
Objective: This study aims to evaluate the efficacy of two free-breathing magnetic resonance imaging (MRI) sequences-spiral ultrashort echo time (spiral UTE) and radial volumetric interpolated breath-hold examination (radial VIBE).
Methods: Patients were prospectively enrolled between February 2021 and September 2022. All participants underwent both 3T MRI scanning, utilizing the radial VIBE sequence and spiral UTE sequence, as well as standard chest CT imaging.
Guidelines for Accurate and Precise Stable Isotope Analysis of Calcite, Dolomite, and Magnesite Using a Carbonate Device for Continuous Flow-Isotope Ratio Mass Spectrometry (CF-IRMS).
Rapid Commun Mass Spectrom
March 2025
School of Earth, Environment & Society, McMaster University, Hamilton, Ontario, Canada.
Rationale: Carbonate minerals are one of the most popular samples for an automated sample preparation system for CF-IRMS, such as GasBench II and iso FLOW, but no standardized analytical protocols exist. This study gives guidelines on optimal analytic conditions for carbon and oxygen isotope analysis of Ca-Mg carbonates when using the carbonate-phosphoric acid reaction method.
Methods: Calcite (CaCO-McMaster Carrara), dolomite (CaMg(CO)-MRSI Dolomite), and magnesite (MgCO-ROM Brazil Magnesite) with two grain size fractions (< 74 and 149-250 μm) were reacted with 103% (specific gravity of 1.
A fluidic device for continuous on-line inductive sensing of proteolytic cleavages.
Lab Chip
January 2025
Institute of Translational Medicine, Department of Health Sciences and Technology, ETH Zürich, 8092 Zürich, Switzerland.
Proteases, an important class of enzymes that cleave proteins and peptides, carry a wealth of potentially useful information. Devices to enable routine and cost effective measurement of their activity could find frequent use in clinical settings for medical diagnostics, as well as some industrial contexts such as detecting on-line biological contamination. In particular, devices that make use of readouts involving magnetic particles may offer distinct advantages for continuous sensing because material they release can be magnetically captured downstream and their readout is insensitive to optical properties of the sample.
View Article and Find Full Text PDFA Nationwide Evaluation of Antibiotic Consumption in Kazakhstan from 2019 to 2023.
Antibiotics (Basel)
November 2024
Graduate School of Public Policy, Nazarbayev University, Astana 010000, Kazakhstan.
Background/objectives: There has been a lack of a holistic approach to evaluating antibiotic consumption in Kazakhstan over the past few years using an internationally recognized methodology. Therefore, this study aimed to provide a nationwide evaluation of antibiotic consumption in Kazakhstan during the period 2019-2023.
Methods: Defined daily doses per 1000 inhabitants per day (DIDs) were calculated for systemic antibiotics (J01 code of the Anatomical Therapeutic Chemical Classification System (ATC)) following the methodology established by the Global Antimicrobial Resistance and Use Surveillance System (GLASS-AMC).
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