We report on a smartphone spectrometer for colorimetric biosensing applications. The spectrometer relies on a sample cell with an integrated grating substrate, and the smartphone's built-in light-emitting diode flash and camera. The feasibility of the smartphone spectrometer is demonstrated for detection of glucose and human cardiac troponin I, the latter in conjunction with peptide-functionalized gold nanoparticles.
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http://dx.doi.org/10.1039/c5an02508g | DOI Listing |
J Hazard Mater
November 2024
Deakin University, Institute for Frontier Materials, Geelong 3220, Australia. Electronic address:
Foodborne and waterborne bacterial infections caused by Escherichia coli (E. coli) pose a serious threat to public health and safety. Therefore, there is an urgent need to develop a fast and accurate diagnostic device for early detection and prevention of bacterial contamination.
View Article and Find Full Text PDFBiosens Bioelectron
February 2025
Department of Nutrition and Food Hygiene, School of Public Health, Anhui Medical University, Hefei, 230032, Anhui, PR China. Electronic address:
Accurate detection of multiple pathogens in the early stages of infection is critical for guiding treatment and saving patients' lives, but current methods are still challenged by low sensitivity, poor robustness and long turnaround times. Here, we report a CRISPR/dCas9-based hotspot self-assembling surface-enhanced Raman scattering (SERS) biosensor (called dCasSERS) and its integration with a smartphone to address these challenges. In this design, bacterial DNA was pre-amplified by loop-mediated isothermal amplification (LAMP), and the repeat sequences of the amplicons were recognized by CRISPR/dCas9, providing abundant sites for the assembly of gold nanoparticles (AuNPs) and forming numerous hotspots for SERS analysis.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
March 2025
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
One novel nanofiber membrane was fabricated which could detect Fe with the help of either fluorescence spectrometer or smartphone. Coffee grounds carbon dots (CCDs) were prepared by the solvent-thermal method, followed by the fabrication of CCDs/polyamide 56 (PA56) composite nanofiber membrane through electrospinning process. The 4 % CCDs/PA56 composite nanofiber membrane (FNM4) maintained good fluorescence performance (λ = 554 nm, λ = 470 nm) even after 5-runs quenching-recovery cycles.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
February 2025
Department of Electrical and Electronic Engineering, Khulna University of Engineering & Technology, Khulna 9203, Bangladesh. Electronic address:
Nanoscale
November 2024
Department of Physics, University of Aveiro, 3810-193 Aveiro, Portugal.
Luminescence thermometry presents precise remote temperature measurement capabilities but faces significant challenges in real-world applications, primarily stemming from the calibration's susceptibility to environmental factors. External factors can compromise accuracy, necessitating resilient measurement protocols to ensure dependable temperature (T) readings across various settings. We explore a novel three-dimensional (3D) approach based on time-gated (t) luminescence thermometric parameters, (,), employing physical mixtures of surface-engineered carbon dots (CDs) based on dibenzoylmethane and rhodamine B.
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