AI Article Synopsis
- Mitochondrial DNA (mtDNA) is crucial for the proper development of oocytes (egg cells).
- The study successfully sequenced the complete mtDNA from a single human oocyte using advanced sequencing techniques for the first time.
- Analyzing mutations in oocyte mtDNA could help identify genetic variations that impact the quality of oocytes.
Article Abstract
Mitochondrial DNA (mtDNA) plays a key role in the development of a competent oocyte. In this study, the complete mtDNA sequence obtained for the first time by multiple displacement amplification approach in combination with next-generation sequencing from a single human oocyte is reported (GenBank accession no. KT364276). The analysis of oocyte mitochondrial mutations could provide a better understanding of the genetic variants correlated with the oocyte quality.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3109/19401736.2015.1115499 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
METTL7A improves bovine IVF embryo competence by attenuating oxidative stress.
Biol Reprod
January 2025
Department of Animal Sciences, Genetics Institute, University of Florida, Gainesville, FL, USA.
In vitro fertilization (IVF) is a widely used assisted reproductive technology to achieve a successful pregnancy. However, the acquisition of oxidative stress in embryo in vitro culture impairs its competence. Here, we demonstrated that a nuclear coding gene, methyltransferase-like protein 7A (METTL7A), improves the developmental potential of bovine embryos.
View Article and Find Full Text PDFFungal-derived tRNAs are expressed and aminoacylated in orchid mitochondria.
Mol Biol Evol
January 2025
Department of Biology, Colorado State University, Fort Collins, CO, USA.
Plant mitochondrial genomes (mitogenomes) experience remarkable levels of horizontal gene transfer (HGT), including the recent discovery that orchids anciently acquired DNA from fungal mitogenomes. Thus far, however, there is no evidence that any of the genes from this interkingdom HGT are functional in orchid mitogenomes. Here, we applied a specialized sequencing approach to the orchid Corallorhiza maculata and found that some fungal-derived tRNA genes in the transferred region are transcribed, post-transcriptionally modified, and aminoacylated.
View Article and Find Full Text PDFPhylogeny of spp. (Decapoda, Pleocyemata) from Peru based on mitochondrial and nuclear data reveals a species complex comprising (Bouvier, 1895) and Holthuis, 1950.
Zookeys
January 2025
Peruvian University Cayetano Heredia, Av. Honorio Delgado 430, San Martín de Porres, Lima, Peru Peruvian University Cayetano Heredia Lima Peru.
Prawns of the genus represent a highly diverse group of high commercial value worldwide. Eight species have been reported from the Peruvian Pacific slope, but their phylogenetic relationships are still unknown. To investigate the systematics of species from Peru, morphological identification and molecular data from nucleotide sequences of three genes were used: cytochrome oxidase subunit I, 16S rRNA, and 28S rRNA.
View Article and Find Full Text PDFNovel factors of cisplatin resistance in epithelial ovarian tumours.
Adv Med Sci
January 2025
Department of Medical Biology, Faculty of Medicine, Pavol Jozef Šafárik University, Košice, Slovak Republic. Electronic address:
Ovarian tumours are these days one of the biggest oncogynecological problems. In addition to surgery, the treatment of ovarian cancer includes also chemotherapy in which platinum preparations are one of the most used chemotherapeutic drugs. The principle of antineoplastic effects of cisplatin (cis-diamminedichloroplatinum(II), CDDP) is its binding to the DNA and the formation of adducts.
View Article and Find Full Text PDFCircadian clockwork controls the balance between mitochondrial turnover and dynamics: What is life … without time marking?
Biochim Biophys Acta Bioenerg
January 2025
Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy. Electronic address:
Circadian rhythms driven by biological clocks regulate physiological processes in all living organisms by anticipating daily geophysical changes, thus enhancing environmental adaptation. Time-resolved serial multi-omic analyses in vivo, ex vivo, and in synchronized cell cultures have revealed rhythmic changes in the transcriptome, proteome, and metabolome, involving up to 50 % of the mammalian genome. Mitochondrial oxidative metabolism is central to cellular bioenergetics, and many nuclear genes encoding mitochondrial proteins exhibit both circadian and ultradian oscillatory expression.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!