AI Article Synopsis
- Researchers found that using microwaves can significantly speed up reactions catalyzed by specific enzymes, leading to quick synthesis of nucleic acids in just 10-50 seconds.
- Without microwave assistance, the same reactions did not produce the desired results in the same timeframe, highlighting the efficiency of microwave irradiation.
- The products created via microwave methods were identical to those made through traditional methods, suggesting that this rapid approach could serve as an effective replacement for conventional nucleic acid synthesis techniques.
Article Abstract
Herein we report microwave-induced enhancement of the reactions catalyzed by Escherichia coli DNA polymerase I and avian myeloblastosis virus-reverse transcriptase. The reactions induced by microwaves result in a highly selective synthesis of nucleic acids in 10-50 seconds. In contrast, same reactions failed to give desired reaction products when carried out in the same time periods, but without microwave irradiation. Each of the reactions was carried out for different duration of microwave exposure time to find the optimum reaction time. The products produced by the respective enzyme upon microwave irradiation of the reaction mixtures were identical to that produced by the conventional procedures. As the microwave-assisted reactions are rapid, microwave could be a useful alternative to the conventional and time consuming procedures of enzymatic synthesis of nucleic acids.
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| http://dx.doi.org/10.1080/15257770.2016.1163383 | DOI Listing |
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